| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ribosome-recycling factor; mitochondrial; RRF; Ribosome-releasing factor; mitochondrial |
| Entrez GeneID | 92399; |
| WB Predicted band size | 28kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human MRRF. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRRF抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*Mitochondrial ribosome recycling factor (MRRF) mutations cause severe neurodegenerative disease with impaired mitochondrial translation*
**作者**：Lake NJ, et al.
**摘要**：该研究通过全外显子测序发现MRRF基因突变与线粒体脑肌病相关。研究者使用特异性MRRF抗体进行免疫印迹和免疫荧光实验，证明突变导致患者细胞中MRRF蛋白表达显著下降，并破坏线粒体核糖体的稳定性，最终影响线粒体蛋白质合成功能。
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2. **文献名称**：*The role of MRRF in mitochondrial ribosome quality control and neurodegeneration*
**作者**：Richman TR, et al.
**摘要**：本文利用MRRF抗体在小鼠模型中探究MRRF蛋白在线粒体核糖体质量控制中的作用。通过免疫共沉淀和蛋白质组学分析，发现MRRF与线粒体核糖体亚基相互作用，并参与错误翻译产物的清除，其功能缺失导致神经元退行性病变。
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3. **文献名称**：*Antibody-based profiling of mitochondrial translation factors in human cell lines*
**作者**：Suzuki T, et al.
**摘要**：研究通过多种线粒体翻译因子抗体(包括MRRF抗体)系统分析人类细胞系中线粒体蛋白质合成机制。结果显示MRRF在HeLa细胞中高表达，且其亚细胞定位严格限于线粒体基质，为相关疾病的诊断标志物开发提供依据。
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**备注**：以上文献为示例性质，实际引用需根据具体研究内容匹配。建议通过PubMed或Web of Science以“MRRF antibody”或“MRRF mitochondrial”为关键词检索最新文献。
MRRF (Mitochondrial Ribosome Recycling Factor), also known as mtRRF, is a critical protein involved in mitochondrial translation. It facilitates ribosome recycling by dissociating ribosomes from mRNA after translation termination, ensuring efficient protein synthesis within mitochondria. This process is essential for maintaining mitochondrial function, particularly in energy production via oxidative phosphorylation.
MRRF antibodies are research tools developed to study the expression, localization, and interactions of MRRF in cellular pathways. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate mitochondrial translation mechanisms. Dysregulation of MRRF has been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson's disease), and cancer, as impaired ribosome recycling disrupts mitochondrial protein synthesis and cellular energy balance.
Recent studies highlight MRRF's role in stress responses and its interaction with mitochondrial translation elongation factors. Mutations in the MRRF gene are associated with combined oxidative phosphorylation deficiencies, underscoring its clinical relevance. Researchers utilize MRRF antibodies to explore these pathological mechanisms and potential therapeutic targets. Commercial MRRF antibodies are typically raised against specific epitopes (human or mouse-derived) and validated for species cross-reactivity and application-specific performance. Ongoing research aims to clarify MRRF's regulatory networks and its implications in aging and metabolic diseases.
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