| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CPP32; SCA-1; CPP32B;Caspase 3;Caspase3 |
| Entrez GeneID | 836 |
| clone | 3D4D10 |
| WB Predicted band size | 31.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human CASP3 (AA: 29-175) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ARHGEF2抗体的参考文献示例(注：以下文献信息为虚构示例，仅用于格式参考)：
1. **文献名称**: "ARHGEF2 regulates cytoskeletal dynamics in migrating endothelial cells"
**作者**: Hanna S, et al.
**摘要**: 本研究通过免疫荧光和Western blot技术，利用ARHGEF2抗体揭示了其在血管内皮细胞迁移中的作用，表明ARHGEF2通过激活RhoA信号通路调控细胞骨架重组。
2. **文献名称**: "ARHGEF2-mediated Rho signaling promotes tumor cell invasion"
**作者**: Li X, et al.
**摘要**: 该文献使用ARHGEF2特异性抗体进行免疫组化和功能实验，证明ARHGEF2在乳腺癌细胞中高表达，并通过调节Rho-ROCK通路增强肿瘤细胞的侵袭能力。
3. **文献名称**: "Interaction of ARHGEF2 with viral proteins during infection"
**作者**: Amano Y, et al.
**摘要**: 通过免疫共沉淀(Co-IP)和ARHGEF2抗体验证，研究发现流感病毒蛋白NS1与ARHGEF2结合，干扰宿主细胞骨架稳定性以促进病毒复制。
4. **文献名称**: "Developmental role of ARHGEF2 in neural crest cell migration"
**作者**: Watanabe T, et al.
**摘要**: 利用ARHGEF2抗体进行胚胎组织染色，发现其在神经嵴细胞迁移中的关键作用，基因敲除导致细胞极性缺陷和发育异常。
(注：实际文献需通过PubMed/Google Scholar等平台检索关键词“ARHGEF2 antibody”或“ARHGEF2 function”获取。)
The ARHGEF2 antibody is a research tool used to detect and study ARHGEF2 (Rho/Rac Guanine Nucleotide Exchange Factor 2), a protein encoded by the *ARHGEF2* gene. Also known as GEF-H1. ARHGEF2 belongs to the RhoGEF family, which regulates Rho GTPases like RhoA, Rac1. and Cdc42 by catalyzing the exchange of GDP for GTP. These GTPases are critical in cytoskeletal reorganization, cell migration, and signaling pathways. ARHGEF2 is implicated in diverse cellular processes, including cell polarity, mitosis, and stress fiber formation, often acting as a molecular switch in response to mechanical or biochemical stimuli.
ARHGEF2 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to analyze protein expression, localization, and interactions. Dysregulation of ARHGEF2 has been linked to pathological conditions, including cancer metastasis, neurological disorders, and inflammatory diseases, making its study clinically relevant. For example, elevated ARHGEF2 levels are associated with increased invasiveness in certain cancers, while mutations may disrupt neural development. Researchers also explore its role in endothelial barrier function and immune responses. Commercial ARHGEF2 antibodies are typically validated for specificity and sensitivity across human, mouse, and rat samples, though performance may vary by application. Proper controls are essential to ensure accurate interpretation, given potential cross-reactivity with homologous proteins.
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