| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARH5; guanine nucleotide regulatory protein TIM; Oncogene TIM; p60 TIM; Rho guanine nucleotide exchange factor 5 |
| Entrez GeneID | 7984; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human ARHGEF5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ARHGEF5抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*ARHGEF5 promotes tumor malignancy and epithelial-mesenchymal transition in hepatocellular carcinoma via activation of STAT3*
**作者**：Li Y, et al.
**摘要**：该研究通过Western blot和免疫组化实验，利用ARHGEF5特异性抗体证实其在肝癌组织中的高表达，并发现其通过激活STAT3信号通路促进肿瘤侵袭和上皮-间质转化(EMT)。抗体验证包括siRNA敲低后的蛋白表达水平对比。
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2. **文献名称**：*RhoGEF-specific antibodies reveal spatiotemporal regulation of RhoA activity in primary endothelial cells*
**作者**：Smith J, et al.
**摘要**：本研究开发了针对多种RhoGEF家族蛋白(包括ARHGEF5)的特异性抗体，用于检测内皮细胞中RhoA的活性调控。通过免疫荧光和Co-IP实验，证明ARHGEF5抗体可特异性识别内源性蛋白，并揭示其在血管生成中的作用。
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3. **文献名称**：*ARHGEF5 interacts with the mTOR pathway to regulate cell proliferation in triple-negative breast cancer*
**作者**：Chen L, et al.
**摘要**：研究利用ARHGEF5抗体进行免疫沉淀和质谱分析，发现其与mTOR通路蛋白相互作用，促进三阴性乳腺癌细胞增殖。抗体的特异性通过CRISPR-Cas9基因敲除实验验证，并用于评估临床样本中ARHGEF5的表达与预后关联。
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注：以上文献为虚构示例，实际研究中建议通过PubMed或Google Scholar以“ARHGEF5 antibody”或“ARHGEF5 function”为关键词检索真实文献。
The ARHGEF5 antibody is a research tool designed to detect and study the ARHGEF5 protein, a member of the Rho guanine nucleotide exchange factor (RhoGEF) family. ARHGEF5. also known as GEF5 or TIM, activates Rho GTPases such as RhoA, RhoB, and RhoC by facilitating the exchange of GDP for GTP, thereby regulating downstream signaling pathways. These pathways are critical for cytoskeletal reorganization, cell migration, adhesion, and proliferation. Dysregulation of ARHGEF5 has been implicated in cancer progression, neurodegenerative disorders, and fibrotic diseases, making it a target of interest in both basic and translational research.
ARHGEF5 antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and interactions in cellular or tissue samples. Researchers employ these antibodies to explore ARHGEF5's role in disease mechanisms, particularly its overexpression in cancers (e.g., lung, breast) where it promotes invasiveness and metastasis. Specific antibody clones, often validated via knockout cell lines or siRNA silencing, ensure minimal cross-reactivity with related RhoGEFs. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary to suit experimental needs. By enabling precise detection of ARHGEF5. these antibodies contribute to understanding its regulatory networks and potential as a therapeutic target or biomarker.
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