| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARHGC; ARHGEF12; Leukemia-associated RhoGEF; RHO guanine nucleotide exchange factor (GEF) 12 (LARG); Rho guanine nucleotide exchange factor 12 |
| Entrez GeneID | 23365; |
| WB Predicted band size | 173kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human ARHGEF12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于ARHGEF12抗体的3篇参考文献示例(注：内容为模拟概括，非真实文献)：
1. **文献名称**：*ARHGEF12 regulates hepatocellular carcinoma invasion via RhoA/ROCK signaling*
**作者**：Zhang Y, et al.
**摘要**：研究利用ARHGEF12抗体检测其在肝癌组织中的高表达，揭示其通过激活RhoA/ROCK通路促进肿瘤细胞迁移和侵袭的机制。
2. **文献名称**：*ARHGEF12 modulates neuronal axon guidance through G protein-coupled receptor signaling*
**作者**：Saito T, et al.
**摘要**：通过免疫组化(ARHGEF12抗体标记)发现其在神经元发育中的分布特征，并证明其通过Gα12/13调控轴突导向的分子路径。
3. **文献名称**：*ARHGEF12 as a potential biomarker in leukemia: Insights from antibody-based profiling*
**作者**：Chen L, et al.
**摘要**：使用ARHGEF12抗体分析白血病患者样本，发现其表达水平与疾病进展相关，可能成为治疗靶点或预后标志物。
(注：以上文献为示例，实际引用需查询PubMed等数据库获取真实信息。)
The ARHGEF12 antibody is a research tool designed to detect and study ARHGEF12 (Rho Guanine Nucleotide Exchange Factor 12), a protein encoded by the ARHGEF12 gene in humans. ARHGEF12. also known as LARG (Leukemia-associated RhoGEF), belongs to the RhoGEF family, which regulates Rho GTPases—key mediators of cytoskeletal dynamics, cell motility, and signal transduction. ARHGEF12 contains a Dbl homology (DH) domain responsible for catalyzing GTP/GDP exchange on RhoA, and a PDZ domain enabling protein-protein interactions. Dysregulation of ARHGEF12 has been implicated in cancers, cardiovascular diseases, and neurological disorders due to its role in RhoA-mediated pathways affecting cell proliferation, migration, and gene expression.
The ARHGEF12 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and function in cellular and tissue samples. Its specificity is validated through knockdown/knockout controls or peptide-blocking assays. Researchers employ this antibody to explore ARHGEF12's involvement in disease mechanisms, such as its oncogenic potential in leukemia or its role in hypertension via RhoA/ROCK signaling. Recent studies also link ARHGEF12 variants to neurodevelopmental disorders, highlighting its broader biological significance.
As a critical reagent, the ARHGEF12 antibody aids in unraveling molecular pathways and potential therapeutic targets, making it valuable in both basic and translational research contexts.
×
