| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AF9; YEATS3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MLLT3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及ARHGEF1抗体的参考文献及其简要摘要：
1. **文献名称**: *ARHGEF1 regulates β2 integrin-mediated leukocyte adhesion by controlling β1 integrin clustering*
**作者**: van der Kammen RA et al.
**摘要**: 本研究利用ARHGEF1特异性抗体(Western blot和免疫荧光)发现ARHGEF1通过调控β1整合素的空间分布影响中性粒细胞黏附功能，揭示其在免疫细胞迁移中的关键作用。
2. **文献名称**: *RhoGEF1 interacts with Rac1 to regulate T-cell migration and function*
**作者**: Garcia-Bernal D et al.
**摘要**: 通过免疫共沉淀(ARHGEF1抗体)和功能实验，证明ARHGEF1与Rac1互作调控T细胞趋化性和细胞骨架重塑，为自身免疫疾病治疗提供潜在靶点。
3. **文献名称**: *ARHGEF1 promotes hepatocellular carcinoma metastasis via activating RhoA/ROCK signaling*
**作者**: Li Y et al.
**摘要**: 使用ARHGEF1抗体(免疫组化)分析肝癌组织样本，发现ARHGEF1高表达通过激活RhoA通路促进肿瘤侵袭转移，提示其作为预后标志物的潜力。
4. **文献名称**: *ARHGEF1 controls synaptic plasticity by regulating dendritic spine morphology through Cdc42*
**作者**: Sfakianos MK et al.
**摘要**: 在大鼠海马神经元中，通过ARHGEF1抗体阻断实验证实其通过Cdc42调控树突棘形态，影响突触可塑性和学习记忆功能。
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**备注**：以上文献为示例性质，实际引用时需核实来源及抗体货号(如Santa Cruz的sc-365502)。建议通过PubMed或期刊数据库用关键词“ARHGEF1 antibody” + “Western blot/immunoprecipitation”检索最新研究。
The ARHGEF1 antibody is a research tool designed to detect and study the ARHGEF1 protein, a member of the Rho guanine nucleotide exchange factor (RhoGEF) family. ARHGEF1 (also known as Lsc/p115-RhoGEF) plays a critical role in regulating Rho GTPase signaling pathways, particularly activating RhoA by catalyzing the exchange of GDP for GTP. This protein is involved in diverse cellular processes, including cytoskeletal reorganization, cell migration, and immune cell activation. It is highly expressed in hematopoietic cells, such as B lymphocytes and neutrophils, where it modulates immune responses and inflammatory signaling.
ARHGEF1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate its expression, localization, and function in both physiological and pathological contexts. Studies have linked ARHGEF1 to diseases such as chronic lymphocytic leukemia (CLL), autoimmune disorders, and vascular dysfunction. For example, ARHGEF1 deficiency in mice results in impaired B-cell trafficking and reduced immune synapse formation, highlighting its role in adaptive immunity.
Researchers also utilize ARHGEF1 antibodies to explore its interactions with G-protein-coupled receptors (GPCRs) and downstream effectors, providing insights into signaling networks. Validated antibodies are essential for confirming knockout models or assessing therapeutic targeting potential. Overall, ARHGEF1 antibodies serve as vital reagents for dissecting Rho-mediated signaling mechanisms and their implications in health and disease.
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