| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L; PCAF-associated factor 65 beta; PAF65-beta; TAF5L; PAF65B |
| Entrez GeneID | 27097; |
| WB Predicted band size | 66kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human TAF5L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TAF5L抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
1. **文献名称**：*TAF5L integrates TFIID subunits in a distinct complex required for embryonic stem cell differentiation*
**作者**：Trowitzsch S, et al.
**摘要**：研究利用TAF5L抗体进行免疫共沉淀和ChIP-seq分析，揭示TAF5L在胚胎干细胞分化中通过调控特定转录复合物(TIF-ID)的组装，影响多能性基因表达。
2. **文献名称**：*Structural insights into the role of TAF5L in RNA polymerase II transcription initiation*
**作者**：Wang HW, et al.
**摘要**：通过TAF5L抗体的Western blot和冷冻电镜技术，解析TAF5L在RNA聚合酶II转录起始复合物中的结构功能，证明其与TAF6的互作对TFIID稳定性至关重要。
3. **文献名称**：*Differential roles of TAF5L and TAF5 in p53-dependent transcriptional regulation*
**作者**：Li Y, et al.
**摘要**：研究使用TAF5L抗体进行免疫荧光和RNA干扰实验，发现TAF5L选择性参与p53靶基因的激活，区别于TAF5.为癌症中转录调控异质性提供机制解释。
**备注**：以上文献为模拟示例，实际检索建议通过PubMed/Google Scholar以“TAF5L antibody”或“TAF5L function”为关键词查找近期研究。
The TAF5L antibody is a research tool used to detect TAF5L (TATA-box binding protein-associated factor 5-like), a subunit of the TFIID complex, which plays a critical role in RNA polymerase II-mediated transcription initiation. TAF5L shares structural and functional similarities with TAF5. a core component of TFIID, and is involved in regulating gene expression by facilitating the assembly of the transcriptional preinitiation complex. Studies suggest TAF5L may also participate in chromatin remodeling and epigenetic regulation, particularly in embryonic development and cellular differentiation processes.
Antibodies targeting TAF5L are widely utilized in molecular biology research to investigate its expression, localization, and interactions within transcriptional machinery. Applications include Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence. Dysregulation of TAF5L has been implicated in certain cancers and developmental disorders, making it a focus in studies exploring transcriptional dysfunctions. Researchers employ TAF5L antibodies to elucidate its role in diseases and its interplay with other transcription factors or coactivators. Commercial TAF5L antibodies are typically validated for specificity and affinity, often through knockout/knockdown controls, ensuring reliability in experimental models. Its study contributes to broader insights into gene regulation mechanisms and therapeutic targeting of transcription-related pathologies.
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