| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SIP-1; SIP1; Smad interacting-protein 1; Smad-interacting 1; SMADIP1 |
| Entrez GeneID | 9839; |
| WB Predicted band size | 136kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human ZEB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZEB2抗体的3篇参考文献示例：
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1. **文献名称**：*ZEB2 regulates endocrine cell differentiation during pancreas development*
**作者**：Frank, D.B. et al.
**摘要**：本研究利用ZEB2特异性抗体，通过免疫组化和Western blot技术，揭示了ZEB2在胰腺内分泌细胞分化中的关键作用，表明其缺失会导致发育异常。
2. **文献名称**：*ZEB2 drives epithelial-mesenchymal transition in colorectal cancer metastasis*
**作者**：Zhang, P. et al.
**摘要**：通过ZEB2抗体染色及功能实验，文章证明ZEB2通过调控EMT相关分子促进结直肠癌侵袭转移，为靶向治疗提供依据。
3. **文献名称**：*Characterization of a novel ZEB2 monoclonal antibody for immunohistochemical analysis*
**作者**：Sato, K. et al.
**摘要**：该研究开发并验证了一种高特异性ZEB2单克隆抗体，优化了其在人组织样本(如肿瘤和胚胎组织)中的免疫组化应用条件。
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*注：以上文献为示例，实际引用时需核实数据库(如PubMed)中的真实文献信息。若需具体文章，建议通过关键词“ZEB2 antibody”、“ZEB2 immunohistochemistry”进一步检索。*
The ZEB2 antibody is a research tool used to detect and study the Zinc Finger E-Box Binding Homeobox 2 (ZEB2) protein, a transcription factor critical in embryonic development and cellular processes. ZEB2. also known as SIP1 (Smad Interacting Protein 1), belongs to the ZEB family of proteins and contains zinc finger domains that enable DNA binding and protein interactions. It primarily regulates epithelial-to-mesenchymal transition (EMT) by repressing epithelial markers (e.g., E-cadherin) and promoting mesenchymal traits, influencing cell migration, differentiation, and tissue morphogenesis.
ZEB2 is implicated in neural crest development, organogenesis, and immune cell regulation. Dysregulation of ZEB2 is linked to pathologies, including cancers (e.g., promoting metastasis in carcinomas) and Mowat-Wilson syndrome, a rare genetic disorder caused by ZEB2 mutations, characterized by developmental delays and congenital anomalies.
Antibodies targeting ZEB2 are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and interactions in tissues or cell lines. These antibodies aid in studying ZEB2’s role in disease mechanisms, therapeutic targeting, and biomarker discovery. Researchers validate their specificity using knockout controls or siRNA to ensure accurate detection, given ZEB2’s structural similarities to ZEB1. Commercial ZEB2 antibodies are available from multiple suppliers, often with species-specific reactivity (e.g., human, mouse, rat). Their application spans developmental biology, oncology, and genetic disorder research.
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