| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin G-associated kinase; EC 2.7.11.1; kinase GAK; |
| Entrez GeneID | 2580; |
| WB Predicted band size | 144kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from Internal of human GAK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GAK(Cyclin G-Associated Kinase)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Cyclin G-associated kinase regulates protein membrane localization by phosphorylating the coat complex AP-1*
**作者**: Zhang J, et al.
**摘要**: 该研究揭示了GAK通过磷酸化AP-1复合物调控细胞内膜运输的机制。研究者利用GAK特异性抗体进行免疫共沉淀和免疫荧光实验，证明GAK缺陷会导致AP-1功能异常，影响溶酶体酶分选及神经突触囊泡运输。
2. **文献名称**: *GAK mediates EGFR signaling to promote hepatitis C virus entry*
**作者**: Cheng X, et al.
**摘要**: 本文发现GAK在丙型肝炎病毒(HCV)入侵宿主细胞过程中起关键作用。通过Western blot和免疫组化实验(使用GAK抗体验证蛋白表达)，作者证明GAK与表皮生长因子受体(EGFR)协同调控病毒内吞，抑制GAK可阻断病毒入侵。
3. **文献名称**: *A genome-wide CRISPR screen identifies novel host targets of SARS-CoV-2 infection*
**作者**: Zhu Y, et al.
**摘要**: 该研究通过全基因组CRISPR筛选发现GAK是新冠病毒(SARS-CoV-2)感染的关键宿主因子。利用GAK抗体进行蛋白定位分析，发现病毒通过劫持GAK介导的网格蛋白依赖的内吞途径进入细胞，提示GAK可作为潜在抗病毒靶点。
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**备注**：以上文献信息为示例性内容，实际研究中建议通过PubMed或Google Scholar检索具体文献。若需实验方法类文献，可补充关键词“antibody validation”或“antibody application”进一步筛选。
**Background of GAK Antibody**
GAK (Cyclin G-Associated Kinase), also known as auxilin-2. is a ubiquitously expressed serine/threonine kinase involved in diverse cellular processes, including clathrin-coated vesicle trafficking, receptor-mediated endocytosis, and cell cycle regulation. It interacts with cyclin G and DNAJA1/Hsc70. playing a critical role in uncoating clathrin-coated vesicles during endocytosis. GAK also modulates the Hippo and TGF-β signaling pathways, impacting cell proliferation, differentiation, and apoptosis.
Dysregulation of GAK has been linked to multiple diseases. Notably, genome-wide association studies (GWAS) identified GAK as a susceptibility locus for Parkinson’s disease, potentially due to its role in lysosomal function and α-synuclein clearance. Additionally, GAK overexpression is observed in certain cancers, correlating with tumor progression and drug resistance, making it a potential therapeutic target.
GAK antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate GAK’s involvement in cellular mechanisms or disease contexts. Both monoclonal and polyclonal antibodies targeting specific epitopes or phosphorylation sites are available, often validated for specificity across human, mouse, or rat models. Research utilizing GAK antibodies continues to unravel its complex roles in health and disease.
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