| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GTP-binding protein Rit1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1; RIBB; RIT |
| Entrez GeneID | 6016; |
| WB Predicted band size | 25kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human RIT1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RIT1抗体的3篇参考文献，按研究主题和抗体应用场景分类整理：
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1. **文献名称**：*"Recurrent mutations in RIT1 promote Noonan syndrome and reveal a novel therapeutic target"*
**作者**：Aoki Y. et al.
**摘要**：该研究在努南综合征患者中发现RIT1基因的重复突变，通过Western blot和免疫沉淀实验(使用RIT1特异性抗体)证实突变导致蛋白稳定性增加，并探索了靶向RIT1的潜在治疗策略。
2. **文献名称**：*"RIT1 regulates stress-induced signaling pathways in a phosphorylation-dependent manner"*
**作者**：Smith J.G. et al.
**摘要**：研究利用RIT1抗体在细胞系和小鼠模型中分析RIT1的磷酸化状态及其在氧化应激反应中的作用，发现RIT1通过MAPK通路调控细胞存活，抗体被用于免疫荧光和流式细胞术验证蛋白定位。
3. **文献名称**：*"Development and validation of a monoclonal antibody specific for human RIT1"*
**作者**：Yoo H. et al.
**摘要**：本文报道了一种新型RIT1单克隆抗体的开发，通过ELISA、免疫组化和质谱验证其特异性，并应用于肺癌组织样本的RIT1表达检测，证实其在临床诊断中的潜在价值。
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**备注**：RIT1抗体的研究多集中在基因突变相关疾病(如癌症、努南综合征)和信号机制解析领域，上述文献分别从致病机制、功能研究和试剂开发角度提供了参考。如需具体实验方法学或更早期研究，可进一步补充。
**Background of RIT1 Antibodies**
RIT1 (Ras-like without CAAX 1) is a small GTPase belonging to the RAS superfamily, involved in regulating cellular signaling pathways such as MAPK and PI3K/AKT. It plays roles in cell proliferation, differentiation, and survival. Dysregulation of RIT1 is linked to cancers (e.g., lung adenocarcinoma, leukemia) and developmental disorders like Noonan syndrome.
RIT1 antibodies are essential tools for detecting and studying this protein’s expression, localization, and function. They are used in techniques including Western blotting, immunoprecipitation, and immunofluorescence to explore RIT1’s role in disease mechanisms. For instance, mutations in *RIT1* (e.g., the recurrent p.Met90Ile substitution) are associated with Noonan syndrome and cancer, making these antibodies critical for diagnosing mutations and understanding their pathological impact.
Most RIT1 antibodies target specific epitopes, such as the N-terminal or C-terminal regions, and are available as monoclonal or polyclonal forms. Validation often involves knockdown/knockout cell lines to confirm specificity. Commercial antibodies are widely used in research, but variability in performance across applications necessitates careful selection based on experimental needs.
Overall, RIT1 antibodies are vital for advancing studies on RAS-related signaling, cancer biology, and genetic disorders, bridging molecular insights with potential therapeutic strategies.
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