Rabbit Polyclonal RCC1 Antibody

The Regulator of Chromosome Condensation 1 (RCC1) is a highly conserved protein that plays a critical role in cell cycle regulation, particularly during mitosis. As the only known guanine nucleotide exchange factor (GEF) for the Ran GTPase, RCC1 is essential for maintaining the Ran-GTP gradient across the nuclear envelope, which governs nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation. RCC1 binds chromatin via interaction with histones and is enriched at regions of active transcription, linking its function to epigenetic regulation. Antibodies targeting RCC1 are widely used in research to study its localization, expression dynamics, and molecular interactions during cell division and interphase. These tools enable techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Dysregulation of RCC1 has been implicated in cancer, as abnormal expression correlates with genomic instability and poor prognosis in malignancies like leukemia and solid tumors. RCC1 antibodies also aid in investigating its role in viral infections, where pathogens may exploit Ran-dependent pathways. Commercial RCC1 antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown, with common epitopes located in the N-terminal region. Their applications span basic cell biology, cancer research, and drug discovery.