| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | POTASSIUM CHANNEL; VOLTAGE-GATED; SHAW-RELATED SUBFAMILY; MEMBER 2; |
| Entrez GeneID | 3747; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Potassium Channel Kv3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **Potassium Channel Kv3.2b 抗体** 的参考文献示例(内容基于既往研究归纳，非真实文献)：
1. **"Differential Subcellular Localization of Kv3.2b in Mammalian Brain Neurons"**
- **作者**: Hernandez-Pineda, R., et al.
- **摘要**: 该研究通过特异性Kv3.2b抗体结合免疫组化技术，揭示了Kv3.2b通道蛋白在啮齿类动物前脑神经元中的亚细胞分布特征，表明其在轴突初始段和突触末梢富集，可能参与调节高频动作电位传递。
2. **"Antibody Validation and Functional Role of Kv3.2b in Auditory Brainstem Circuits"**
- **作者**: Li, W., & Trussell, L.O.
- **摘要**: 研究利用Kv3.2b特异性抗体进行Western blot和免疫荧光验证，证明其在听觉脑干核团(如内侧上橄榄核)中的高表达，并通过电生理学实验证明Kv3.2b对神经元快速复极化的关键作用。
3. **"Developmental Expression of Kv3.2b in Cortical GABAergic Interneurons"**
- **作者**: Lau, D., et al.
- **摘要**: 通过Kv3.2b抗体标记，研究发现该通道在发育早期皮质GABA能中间神经元中的动态表达模式，提示其对皮层网络同步化发育的潜在影响。
4. **"Kv3.2b Antibody Reveals Altered Expression in Epilepsy Models"**
- **作者**: Sanchez-Aguilera, A., et al.
- **摘要**: 使用Kv3.2b抗体检测癫痫模型小鼠海马区通道表达变化，发现其下调可能导致抑制性中间神经元兴奋性失衡，加剧癫痫样放电。
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*注：以上为模拟文献，实际引用时需查询真实数据库(如PubMed)获取准确信息。*
The potassium channel Kv3.2b, a splice variant of the *KCNC2* gene, belongs to the Shaw-type (Kv3) voltage-gated potassium channel family. It is predominantly expressed in high-frequency firing neurons, including cortical interneurons, brainstem nuclei, and thalamic reticular neurons. Kv3.2b channels play a critical role in regulating action potential repolarization and enabling rapid neuronal firing, essential for sustained high-frequency signaling in circuits involved in auditory processing, sleep-wake cycles, and motor coordination.
Antibodies targeting Kv3.2b are essential tools for studying its expression, localization, and functional roles. These antibodies are typically generated against specific epitopes within the channel's variable C-terminal region, distinguishing Kv3.2b from other Kv3 subtypes (e.g., Kv3.1. Kv3.3). Validated applications include Western blotting, immunohistochemistry, and immunofluorescence, allowing researchers to map Kv3.2b distribution in brain tissues or assess its dysregulation in neurological disorders. For instance, altered Kv3.2b expression has been linked to epilepsy, schizophrenia, and neurodegeneration. Specificity is often confirmed using *Kcnc2* knockout models or siRNA-mediated knockdown. Commercial antibodies may vary in host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal), requiring optimization for tissue fixation and species cross-reactivity. These reagents remain pivotal in elucidating Kv3.2b's contributions to neuronal excitability and disease mechanisms.
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