Rabbit Polyclonal p14ARF Antibody

The p14 ARF (Alternative Reading Frame) protein, encoded by the INK4a/ARF locus on human chromosome 9p21. is a critical tumor suppressor involved in cell cycle regulation and apoptosis. This locus uniquely encodes two distinct proteins, p16 INK4a and p14 ARF, through alternative reading frames. While p16 primarily inhibits CDK4/6 to block Rb phosphorylation, p14 ARF stabilizes p53 by binding to MDM2. thereby preventing p53 degradation and enhancing its tumor-suppressive functions. Dysregulation of p14 ARF, through mutations, deletions, or epigenetic silencing, is frequently observed in cancers, contributing to uncontrolled proliferation and evasion of apoptosis. Antibodies targeting p14 ARF are essential tools for studying its expression, localization, and interactions in both normal and pathological contexts. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research on tumorigenesis, therapeutic resistance, and cellular stress responses. Given its role in p53 activation, p14 ARF is also investigated in therapies targeting p53-deficient cancers. However, challenges persist due to its low abundance, shared locus with p16. and sequence overlap, necessitating highly specific antibodies validated across experimental models.