| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Antigen KI-67; MKI67; |
| Entrez GeneID | 4288; |
| WB Predicted band size | 360kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human ki67. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于Ki67抗体的参考文献(示例基于经典研究，具体文献请核实原文)：
1. **文献名称**：*The Ki-67 protein: from the known and the unknown*
**作者**：Scholzen T, Gerdes J
**摘要**：该综述总结了Ki67抗体的生物学功能及其在细胞增殖检测中的应用，讨论了Ki67蛋白在细胞周期中的动态表达模式及其作为肿瘤增殖标志物的临床价值。
2. **文献名称**：*Immunohistochemical detection of Ki67 in breast cancer correlates with transcriptional regulation of genes related to apoptosis and cell death*
**作者**：Isola J, et al.
**摘要**：研究利用Ki67抗体评估乳腺癌组织增殖活性，发现Ki67高表达与肿瘤侵袭性及患者预后不良相关，并揭示了其与凋亡相关基因的潜在调控机制。
3. **文献名称**：*Assessment of Ki67 in breast cancer: recommendations from the International Ki67 in Breast Cancer Working Group*
**作者**：Dowsett M, et al.
**摘要**：国际工作组提出Ki67抗体检测的标准化建议，强调其在乳腺癌分子分型及治疗反应预测中的作用，同时指出实验室间结果差异的挑战。
4. **文献名称**：*Ki67 proliferation index as a tool for chemotherapy decisions in ER+ breast cancer*
**作者**：Nielsen TO, et al.
**摘要**：研究探讨Ki67抗体检测在雌激素受体阳性乳腺癌中的临床实用性，支持其作为化疗决策的辅助生物标志物，尤其在中高风险患者中。
(注：以上为模拟示例，实际文献需通过PubMed等数据库检索确认。)
**Background of the Ki67 Antibody**
The Ki67 antibody is a widely used tool in biomedical research and clinical pathology to assess cell proliferation. The Ki67 protein, first identified in 1983 by Gerdes et al., is a nuclear non-histone antigen expressed in all active phases of the cell cycle (G1. S, G2. M) but absent in quiescent (G0) cells. Its name originates from the city of Kiel (Ki) and the clone number 67.
Structurally, Ki67 is a large protein (~360 kDa) encoded by the *MK167* gene located on chromosome 10. While its exact molecular function remains unclear, it is associated with ribosomal RNA transcription and chromatin organization during mitosis.
Clinically, Ki67 serves as a proliferation marker, with its expression level correlating with tumor aggressiveness and patient prognosis. It is routinely used in cancer diagnostics (e.g., breast cancer, lymphomas) to determine the proliferation index, guiding treatment decisions. High Ki67 levels often indicate poor prognosis and may influence chemotherapy or radiation therapy choices.
In research, Ki67 staining helps study cell proliferation dynamics in development, tissue regeneration, and disease models. Despite its utility, Ki67 has limitations, including variable quantification methods and lack of standardized scoring protocols. Alternatives like PCNA exist, but Ki67 remains preferred for its sensitivity in detecting active proliferation. Ongoing studies aim to better define its biological roles and optimize clinical applications.
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