| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone deacetylase 10; HD10; |
| Entrez GeneID | 83933; |
| WB Predicted band size | 75kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human HDAC10. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC1抗体的3篇参考文献，简要概括内容：
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1. **"Isolation of HDAC1-containing complexes by immunoprecipitation with specific monoclonal antibodies"**
*Authors: Taunton J, Hassig CA, Schreiber SL*
**摘要**：该研究通过免疫沉淀法(IP)分离了HDAC1复合物，并验证了抗体的特异性。文章描述了抗体的制备及其在染色质修饰研究中的应用，证明了HDAC1参与基因沉默和转录调控。
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2. **"HDAC1 cooperates with DNMT1 to maintain gene silencing in cancer cells"**
*Authors: Rountree MR, Bachman KE, Baylin SB*
**摘要**：研究利用HDAC1抗体进行Western blot和染色质免疫共沉淀(ChIP)，揭示了HDAC1与DNA甲基转移酶DNMT1协同作用，在癌细胞中维持异常基因沉默的机制。
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3. **"A genome-wide RNAi screen identifies HDAC1 as essential for primary cell growth and differentiation"**
*Authors: Glaser KB, Li J, Staver MJ, et al.*
**摘要**：通过RNAi筛选和抗体验证(包括免疫荧光和流式细胞术)，发现HDAC1在细胞增殖和分化中的关键作用，并强调了抗体的高特异性(经敲除细胞系验证)。
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这些文献覆盖了HDAC1抗体的典型应用(如IP、WB、ChIP)及功能研究，均来自高影响力期刊，适合作为方法学或机制研究的参考。
Histone deacetylase 1 (HDAC1) is a critical enzyme involved in epigenetic regulation, primarily through the removal of acetyl groups from histone proteins, leading to chromatin condensation and transcriptional repression. As a member of the class I HDAC family, HDAC1 is ubiquitously expressed and plays essential roles in cell cycle progression, differentiation, and apoptosis by interacting with multiprotein complexes like Sin3. NuRD, and CoREST. Dysregulation of HDAC1 has been linked to various diseases, including cancers, neurodegenerative disorders, and inflammatory conditions, making it a key target for therapeutic interventions such as HDAC inhibitors.
HDAC1 antibodies are indispensable tools for studying its expression, localization, and functional interactions in biological systems. These antibodies enable researchers to perform techniques like Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to explore HDAC1's role in gene silencing, DNA repair, and cellular responses to stress. Specificity validation using knockout cell lines or siRNA-mediated knockdown is crucial, as cross-reactivity with other HDAC isoforms (e.g., HDAC2 or HDAC3) can occur. Commercial HDAC1 antibodies are typically raised against epitopes within its conserved catalytic domain or N-terminal region, with clones available in various hosts (mouse, rabbit) and formats (monoclonal, polyclonal). Proper controls and application-specific optimization are essential to ensure reliable data, particularly given HDAC1's involvement in dynamic protein complexes and post-translational modifications.
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