| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ONCOGENE DJ1; PARK7 AMYOTROPHIC LATERAL SCLEROSIS-PARKINSONISM/DEMENTIA COMPLEX 2; PARK7 GENE; |
| Entrez GeneID | 11315; |
| WB Predicted band size | 22kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human DJ-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于DJ-1抗体的模拟参考文献示例(注：文献信息为示例性质，实际文献请通过学术数据库查询)：
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1. **"DJ-1 as a biomarker for Parkinson's disease: Development of a novel monoclonal antibody"**
*Author: Smith A et al.*
摘要：开发了一种高特异性DJ-1单克隆抗体，验证其在人脑组织及脑脊液中的检测能力，证明DJ-1蛋白水平与帕金森病病程相关。
2. **"DJ-1 antibody validation in oxidative stress models"**
*Author: Tanaka K et al.*
摘要：通过Western blot和免疫组化验证DJ-1抗体在细胞氧化应激模型中的特异性，揭示DJ-1在调控线粒体功能中的保护性作用。
3. **"Comparative study of DJ-1 expression in cancer using species-cross-reactive antibodies"**
*Author: Chen L et al.*
摘要：比较不同物种(人、小鼠)中DJ-1蛋白表达水平，利用交叉反应性抗体发现DJ-1在肺癌组织中的异常高表达，提示其作为肿瘤标志物的潜力。
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*注：以上为模拟内容，真实文献建议通过PubMed、Web of Science等平台搜索关键词(如DJ-1 antibody、PARK7 protein)获取。*
DJ-1 antibodies are essential tools for studying the DJ-1 protein (also known as PARK7), a multifunctional protein encoded by the *PARK7* gene located on chromosome 1p36. DJ-1 gained prominence due to its association with autosomal recessive early-onset Parkinson’s disease (PD), as mutations in *PARK7* disrupt its neuroprotective roles. The protein is implicated in oxidative stress response, mitochondrial regulation, and proteasome activity, making it a key focus in neurodegeneration research. DJ-1 antibodies are designed to target specific epitopes, enabling detection of its expression, localization, and post-translational modifications in cellular and animal models.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate DJ-1’s role in PD pathogenesis. For example, they help identify reduced DJ-1 levels or mislocalization in PD patient tissues or toxin-induced PD models. Cross-reactivity with DJ-1 orthologs in common model organisms (e.g., mice, rats) supports translational research. Additionally, DJ-1 antibodies aid in studying its interactions with PD-linked proteins like α-synuclein, LRRK2. and PINK1. shedding light on disease mechanisms. Commercial DJ-1 antibodies are typically validated for specificity using knockout controls or siRNA knockdown. However, researchers must verify batch consistency, as nonspecific binding can occur due to DJ-1’s structural homology with other proteins (e.g., bacterial proteases). Overall, DJ-1 antibodies remain critical for unraveling its dual roles as a redox-sensitive chaperone and a biomarker in neurodegenerative and cancer-related studies.
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