| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | COL4A1 NC1 domain; COLLAGEN OF BASEMENT MEMBRANE; ALPHA-1 CHAIN ARRESTEN; alpha 1 type IV collagen preproprotein; collagen IV alpha-1 polypeptide |
| Entrez GeneID | 1282; |
| WB Predicted band size | 160kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human Collagen IV. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于 **Collagen IV抗体** 的参考文献摘要(内容经过简化整理)：
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1. **文献名称**：*"Collagen IV Antibodies in the Diagnosis of Glomerular Diseases"*
**作者**：Levy, M., et al.
**摘要**：该研究探讨了抗Collagen IV抗体在肾小球疾病中的诊断价值，通过免疫荧光和免疫组化技术，发现其在Alport综合征和薄基底膜肾病中的特异性表达模式，为遗传性和获得性肾脏疾病的鉴别提供了依据。
2. **文献名称**：*"Mutations in Collagen IV and Autoantibody Profiles in Alport Syndrome"*
**作者**：Hudson, B.G., et al.
**摘要**：研究分析了Alport综合征患者中Collagen IV基因突变与自身抗体的关联，发现抗Collagen IV α3链抗体的存在可能加速肾小球基底膜的损伤，为疾病进展机制提供了新见解。
3. **文献名称**：*"Collagen IV Staining in Tumor Microenvironment Analysis"*
**作者**：Kalluri, R., & Zeisberg, M.
**摘要**：通过抗Collagen IV抗体标记肿瘤基底膜，揭示了其在癌症转移中的作用。研究表明，基底膜完整性的破坏与肿瘤侵袭性相关，为癌症预后评估提供了潜在标志物。
4. **文献名称**：*"Technical Validation of Collagen IV Antibodies in Immunohistochemistry"*
**作者**：Van den Heuvel, L.P., et al.
**摘要**：系统性评估了不同来源的Collagen IV抗体在组织染色中的特异性和敏感性，提出了标准化检测流程，以提高肾脏和皮肤活检诊断的准确性。
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**注**：以上文献为示例性质，实际引用时需根据具体研究领域(如肾脏病理学、肿瘤学或免疫学)选择针对性文献，并通过PubMed/Google Scholar等平台核实详细信息。
Collagen IV is a critical structural protein found in the basement membrane, a specialized extracellular matrix that provides structural support to cells and tissues. It forms a flexible network through self-assembly into a mesh-like scaffold, essential for tissue integrity and cellular interactions. Composed of six distinct α chains (α1–α6), Collagen IV exists primarily as α1α1α2 heterotrimers in most basement membranes, while other combinations (e.g., α3α4α5) are tissue-specific, notably in the kidney, lungs, and cochlea.
Antibodies targeting Collagen IV are widely used in research and diagnostics to study basement membrane architecture and related pathologies. In clinical settings, they help diagnose kidney diseases like Alport syndrome, caused by mutations in α3/α4/α5 chains, and autoimmune disorders such as Goodpasture syndrome, where autoantibodies attack the α3 chain. In cancer research, Collagen IV antibodies assess tumor microenvironment changes, as basement membrane degradation often correlates with metastasis.
These antibodies are also vital tools in techniques like immunofluorescence, immunohistochemistry, and Western blotting to visualize basement membrane distribution and integrity. Monoclonal antibodies offer high specificity for particular α chains, while polyclonal antibodies may detect broader epitopes. Researchers must validate antibody specificity due to potential cross-reactivity between Collagen IV isoforms and species variations. Overall, Collagen IV antibodies bridge structural biology, disease mechanisms, and therapeutic development.
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