| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Collagen alpha 1; Collagen alpha 1(III) chain [Precursor]; |
| Entrez GeneID | 1281; |
| WB Predicted band size | 138kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human collagen III. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Collagen III抗体的3篇参考文献概览(基于公开信息模拟整理，非真实文献)：
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1. **文献名称**：*Collagen III Deposition in Fibrotic Liver and Its Detection by Immunohistochemistry*
**作者**：Smith J, et al.
**摘要**：研究利用抗Collagen III抗体(克隆号：FH-7A)通过免疫组化技术检测肝纤维化模型中的胶原沉积，证实其在早期纤维化中的特异性表达，为疾病诊断提供标记物。
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2. **文献名称**：*Type III Collagen Antibody Validation for Western Blotting in Vascular Remodeling Studies*
**作者**：Lee H, et al.
**摘要**：作者验证了多款抗Collagen III抗体(包括Sigma-Aldrich C7805)在Western blot中的特异性，发现某些抗体与降解产物存在交叉反应，建议结合质谱验证以提高实验准确性。
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3. **文献名称**：*Role of Collagen III in Dermal Wound Healing: Insights from Antibody-Based Localization*
**作者**：Garcia R, et al.
**摘要**：通过抗Collagen III抗体的免疫荧光技术，研究揭示了其在皮肤创伤修复过程中动态分布，表明Collagen III在临时基质形成中起关键作用，促进成纤维细胞迁移。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“Collagen III antibody”、“Type III collagen immunohistochemistry”等获取近期论文。
Collagen III antibody is a crucial tool in biomedical research, primarily targeting collagen type III, a fibrillar collagen abundantly found in elastic tissues such as skin, blood vessels, and internal organs. Collagen III, encoded by the *COL3A1* gene, is a key component of the extracellular matrix (ECM) and plays a vital role in maintaining tissue integrity, elasticity, and structural support. It is often co-expressed with collagen type I, particularly during early stages of wound healing and tissue repair, forming heterotypic fibrils that regulate fibril diameter and mechanical properties.
Antibodies against collagen III are widely used to study its distribution, expression, and pathological alterations in diseases. In research, these antibodies help visualize collagen III via techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. They are essential in investigating fibrotic disorders (e.g., liver fibrosis, pulmonary fibrosis), vascular diseases (e.g., aneurysms, atherosclerosis), and connective tissue abnormalities, such as vascular Ehlers-Danlos syndrome (vEDS), caused by *COL3A1* mutations.
Clinically, collagen III antibodies aid in diagnosing tissue remodeling processes. For instance, elevated collagen III levels in fibrotic tissues or serum markers correlate with disease progression. Additionally, they are used to study developmental biology, as collagen III is critical in embryonic ECM organization. Commercial collagen III antibodies are typically monoclonal or polyclonal, with validation in specific applications. Researchers must consider species reactivity, cross-reactivity with other collagens, and epitope specificity when selecting antibodies to ensure accurate results. Overall, collagen III antibodies remain indispensable for understanding ECM dynamics in health and disease.
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