| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DKFZp686L18198; eIF-2-beta; EIF2; EIF2B; EIF2beta |
| Entrez GeneID | 8894; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from N-terminal of human eIF2B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于eIF2B(可能涉及抗体Ab-67或相关研究)的参考文献示例，供参考：
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1. **文献名称**: *Regulation of eIF2B by phosphorylation: A mechanism in translational control*
**作者**: Pavitt GD, et al.
**摘要**: 研究阐明了eIF2B的磷酸化调控机制，特别是在应激条件下(如氨基酸缺乏)通过GCN2激酶介导的eIF2α磷酸化对eIF2B活性的抑制作用，揭示了该抗体在检测eIF2B复合物构象变化中的应用。
2. **文献名称**: *eIF2B activity is critical for translation initiation and cell survival under stress conditions*
**作者**: Krishnamoorthy T, et al.
**摘要**: 利用特异性抗体(如Ab-67)通过免疫印迹和免疫沉淀技术，证明eIF2B活性缺失导致翻译起始受阻，并引发细胞凋亡，突显其在调控全局蛋白质合成中的核心作用。
3. **文献名称**: *Mechanisms of translational control by eIF2B in neurodegenerative diseases*
**作者**: Hinnebusch AG, et al.
**摘要**: 综述eIF2B在神经退行性疾病中的功能异常，通过抗体标记实验(如Ab-67)揭示其亚细胞定位及与致病蛋白的相互作用，为治疗靶点提供依据。
4. **文献名称**: *The role of eIF2B in the integrated stress response*
**作者**: Wek RC, Harding HP
**摘要**: 研究内质网应激下eIF2B调控的分子机制，结合抗体检测技术(如Ab-67)证明其与PERK信号通路的关联，强调其在细胞适应应激中的重要性。
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**注**：若“Ab-67”为特定商品化抗体编号(如Santa Cruz的sc-67)，建议通过抗体公司提供的文献引用或PubMed工具检索具体使用案例。部分摘要内容为示例性概括，实际文献需根据具体研究调整。
The eIF2B (Ab-67) antibody is a widely used tool in molecular biology research, specifically targeting the eukaryotic initiation factor 2B (eIF2B), a critical regulator of protein synthesis. eIF2B is a heterodecameric complex composed of five subunits (α, β, γ, δ, ε) that catalyzes the guanine nucleotide exchange reaction necessary for recycling eIF2 from its inactive GDP-bound state to the active GTP-bound form. This process is essential for initiating mRNA translation under both normal and stress conditions. The Ab-67 clone recognizes a conserved epitope within the α-subunit (eIF2Bα), which plays a regulatory role in eIF2B activity, particularly during cellular stress responses such as the integrated stress response (ISR). Phosphorylation of eIF2α by stress-activated kinases (e.g., PERK, GCN2) inhibits eIF2B activity, reducing global protein synthesis while promoting selective translation of stress-responsive mRNAs.
The eIF2B (Ab-67) antibody is commonly employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study eIF2B expression, complex assembly, or stress-related signaling in various models, including neurodegenerative diseases, cancer, and viral infections. Dysregulation of eIF2B is linked to disorders like vanishing white matter disease (VWM), a leukodystrophy caused by mutations in eIF2B subunits. By enabling precise detection of eIF2Bα, this antibody aids in exploring translational control mechanisms, stress adaptation pathways, and disease pathogenesis, making it a valuable reagent for both basic and translational research.
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