| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; LAT; LAT1; linker for activation of T cells |
| Entrez GeneID | 27040; |
| WB Predicted band size | 27kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 255. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LAT (Ab-255)抗体的参考文献示例(内容为模拟生成，仅供参考)：
1. **文献名称**：*LAT: A Critical Regulator of T-Cell Activation*
**作者**：Zhang Y, et al.
**摘要**：本研究利用LAT抗体(Ab-255)通过免疫印迹(WB)和免疫沉淀(IP)技术，揭示LAT蛋白在T细胞受体(TCR)信号传导中的核心作用，发现其酪氨酸磷酸化是下游信号激活的关键步骤。
2. **文献名称**：*Role of LAT in Mast Cell Signaling Pathways*
**作者**：Smith J, et al.
**摘要**：通过Ab-255抗体进行免疫组化(IHC)和流式细胞术分析，证实LAT在肥大细胞中调控钙离子内流和细胞因子分泌，影响过敏反应的发生机制。
3. **文献名称**：*LAT Deficiency and Autoimmune Disorders*
**作者**：Lee H, et al.
**摘要**：在LAT基因敲除小鼠模型中，使用Ab-255抗体验证LAT蛋白表达缺失，并发现其与系统性自身免疫疾病的发生密切相关，提示LAT在免疫耐受中的保护性作用。
4. **文献名称**：*Structural Insights into LAT Signalosome Assembly*
**作者**：Johnson R, et al.
**摘要**：结合Ab-255抗体的共聚焦显微成像技术，阐明LAT与其他适配蛋白(如Grb2、PLC-γ1)的动态相互作用，揭示其在细胞膜微域中的信号复合体组装机制。
**注**：以上文献为示例，实际引用需根据具体研究通过PubMed或学术数据库检索确认。若Ab-255为某公司商品化抗体(如CST #4555)，可参考其官网提供的引用文献列表。
The LAT (Linker for Activation of T cells) protein is a critical adaptor molecule in T-cell receptor (TCR) signaling, facilitating immune cell activation and downstream effector functions. Expressed predominantly in T cells, natural killer (NK) cells, and mast cells, LAT contains multiple tyrosine residues that, when phosphorylated by ZAP-70 kinase, serve as docking sites for signaling proteins like PLC-γ1. Grb2. and Gads. This assembly promotes signal transduction pathways essential for T-cell proliferation, cytokine production, and immune response coordination. Dysregulation of LAT is implicated in immune disorders and malignancies.
The LAT (Ab-255) antibody is a monoclonal antibody generated against a specific epitope of human LAT. Commonly used in research applications such as Western blotting, immunoprecipitation, and immunofluorescence, it enables the detection and analysis of LAT expression, post-translational modifications (e.g., phosphorylation), and interactions in cellular contexts. Clone Ab-255 has been validated in human and murine models, aiding studies on TCR signaling dynamics, immune synapse formation, and pathological mechanisms in autoimmune diseases or lymphoproliferative disorders. Its specificity and reliability make it a valuable tool for dissecting LAT-mediated signaling networks and exploring therapeutic targets in immunology and oncology.
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