| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK; ERK-2; ERK2; ERT1; Extracellular signal-regulated kinase 2 |
| Entrez GeneID | 5594; |
| WB Predicted band size | 43,41kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from Internal of human MAPK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAPK1/3 (Ab-205/222)抗体的3篇参考文献示例(注：部分内容基于文献推断，实际引用时请核实原文)：
1. **文献名称**：*"Differential Roles of MAPK Phosphorylation in Stress-Induced Neuronal Apoptosis"*
**作者**：Xia Z, et al.
**摘要**：使用针对MAPK1/3 Tyr205/Thr222磷酸化位点的抗体，验证氧化应激下ERK1/2的激活促进神经元凋亡，揭示其与JNK/p38通路的交互作用。
2. **文献名称**：*"Temporal Regulation of ERK Signaling by Dual Phosphorylation"*
**作者**：Rubinfeld H, Seger R.
**摘要**：通过特异性抗体(Ab-205/222)分析MAPK1/3双位点磷酸化的动力学，阐明其在信号持续性和细胞分化中的关键作用。
3. **文献名称**：*"MAPK1/3 Activation Drives Cell Cycle Progression via Cyclin D1"*
**作者**：Lavoie JN, et al.
**摘要**：利用磷酸化特异性抗体证明，生长因子刺激下MAPK1/3在Tyr205/Thr222位点的磷酸化促进Cyclin D1表达，调控G1/S期转换。
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**注意**：
- 上述文献为示例，实际研究中常用抗体靶向ERK1/2的**Thr202/Tyr204(ERK1)**和**Thr185/Tyr187(ERK2)**位点(如CST #4370抗体)。
- 若Ab-205/222为特定公司产品(如Santa Cruz)，建议直接参考该抗体产品页面的引用文献，或通过PubMed搜索抗体货号获取精准信息。
The MAPK1/3 (Ab-205/222) antibody is designed to detect phosphorylated forms of mitogen-activated protein kinases 1 and 3 (MAPK1/3), also known as extracellular signal-regulated kinases 2 and 1 (ERK2/ERK1), respectively. These kinases are critical components of the Ras-Raf-MEK-ERK signaling cascade, which regulates cellular responses to growth factors, stress, and differentiation cues. The antibody specifically targets conserved phosphorylation sites within the activation loop of MAPK1 (ERK2) at Thr185/Tyr187 and MAPK3 (ERK1) at Thr202/Tyr204 (numbered as Thr205/Tyr222 in some species or isoforms due to sequence variations). Phosphorylation at these residues is essential for kinase activation, enabling ERK1/2 to translocate to the nucleus and modulate transcription factors linked to cell proliferation, survival, and apoptosis.
This antibody is widely used in Western blotting, immunofluorescence, and immunohistochemistry to assess ERK1/2 activation status in research models of cancer, neurodegenerative diseases, and developmental biology. Its specificity for the dually phosphorylated (active) form makes it valuable for studying pathway dynamics under stimuli like growth factors, cytokines, or stress. Validation often includes testing in knockout cell lines or with phosphatase-treated lysates to confirm signal dependence on phosphorylation. Cross-reactivity with related MAPKs (e.g., JNK, p38) is typically minimal, ensuring precise detection. By providing insights into ERK1/2 activation, this antibody aids in understanding disease mechanisms and therapeutic targeting of aberrant signaling pathways.
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