| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADRBK1; adrenergic; beta; receptor kinase 1; ARBK1 |
| Entrez GeneID | 156; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from N-terminal of human ADRBK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及GRK2(Ab-86)抗体的参考文献概述，供参考：
1. **文献名称**：*"GRK2 phosphorylation by ERK regulates cardiac hypertrophy"*
**作者**：Lymperopoulos A. et al.
**摘要**：研究揭示了ERK介导的GRK2磷酸化在心肌肥大中的作用，使用GRK2 (Ab-86)抗体检测心脏组织中GRK2的磷酸化状态，发现其活性与心力衰竭病理相关。
2. **文献名称**：*"Role of GRK2 in dopamine receptor desensitization in Parkinson's models"*
**作者**：Ahmed M.R. et al.
**摘要**：通过GRK2 (Ab-86)抗体验证神经元中GRK2的表达变化，证明其在多巴胺受体脱敏中的作用，提示GRK2抑制剂可能改善帕金森病症状。
3. **文献名称**：*"GRK2 regulates β-arrestin-mediated signaling in cancer metastasis"*
**作者**：Nijboer C. et al.
**摘要**：利用GRK2 (Ab-86)抗体进行免疫印迹和免疫组化分析，发现GRK2通过调控β-arrestin信号通路促进乳腺癌细胞迁移和侵袭。
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注：以上为模拟示例，实际文献需通过PubMed或Google Scholar检索关键词“GRK2 Ab-86 antibody”或“GRK2 phosphorylation site”获取。推荐检查近年的细胞信号转导或疾病机制相关研究。
GRK2 (Ab-86) is a monoclonal antibody specifically targeting G protein-coupled receptor kinase 2 (GRK2), a key regulator of G protein-coupled receptor (GPCR) signaling. GRK2. also known as β-adrenergic receptor kinase 1 (βARK1), phosphorylates activated GPCRs, promoting their desensitization and internalization via β-arrestin binding. This kinase plays critical roles in cardiovascular function, immune responses, and neuronal signaling, with dysregulation linked to heart failure, hypertension, and cancer.
The GRK2 (Ab-86) antibody is commonly used to detect endogenous GRK2 protein levels in human, mouse, and rat samples. It recognizes an epitope within the C-terminal region of GRK2. allowing identification of the full-length protein (~80 kDa) in applications like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity has been validated in knockout controls and functional studies, making it a reliable tool for investigating GRK2 expression patterns, cellular localization, and interaction partners.
Research utilizing this antibody has contributed to understanding GRK2's role in pathological conditions, particularly its upregulation in heart failure models and its impact on β-adrenergic receptor downregulation. It also aids in studying GRK2's non-canonical functions, such as interactions with non-GPCR substrates (e.g., PI3K, HDAC6) in cancer metastasis and inflammation. The antibody's robust performance across species supports translational studies bridging cellular models to disease mechanisms.
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