| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FADD; Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; Fas-associating protein with death domain |
| Entrez GeneID | 14082; |
| WB Predicted band size | 30kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human FADD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FADD(Ab-191)抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis*
**作者**：J. Zhang et al.
**摘要**：本研究首次克隆并鉴定了FADD蛋白，揭示了其通过死亡结构域与Fas受体结合，在凋亡信号转导中的关键作用。实验中利用Ab-191抗体通过免疫共沉淀验证了FADD与Fas的相互作用，证实其参与激活Caspase级联反应。
2. **文献名称**：*Targeted disruption of the FADD gene causes premature embryonic lethality and dysregulated apoptosis*
**作者**：A. M. Chinnaiyan et al.
**摘要**：通过构建FADD基因敲除小鼠模型，研究团队发现FADD缺失导致胚胎早期死亡及细胞凋亡异常。Ab-191抗体被用于Western blot分析，证明FADD缺失后死亡受体信号通路(如TNF-α诱导的凋亡)显著受阻。
3. **文献名称**：*FADD regulates NF-κB activation and colorectal cancer pathogenesis*
**作者**：E. E. Varfolomeev et al.
**摘要**：该文献探讨了FADD在结直肠癌中的双重作用，除凋亡调控外，还通过NF-κB通路影响肿瘤进展。使用Ab-191抗体进行免疫组化，发现FADD在癌组织中的低表达与患者预后不良相关，提示其抑癌潜力。
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**说明**：以上文献为示例，实际引用需根据具体研究内容检索真实发表的论文。FADD相关研究多聚焦于凋亡、炎症及癌症领域，Ab-191作为常用抗体常被用于蛋白表达检测及功能机制验证。
**Background of FADD (Ab-191) Antibody**
The FADD (Fas-associated protein with death domain) antibody, specifically the clone Ab-191. is a widely used tool in apoptosis and necroptosis research. FADD is a critical adaptor protein that mediates cell death signaling through interactions with death receptors (e.g., Fas, TNF-R1. TRAIL-R). It contains a C-terminal death domain (DD) for receptor binding and an N-terminal death effector domain (DED) that recruits caspase-8/10 to form the death-inducing signaling complex (DISC), initiating caspase activation and apoptosis. FADD also participates in non-apoptotic processes, including innate immune responses and cell cycle regulation.
The Ab-191 antibody, typically raised against a synthetic peptide corresponding to human FADD, recognizes endogenous FADD across species (human, mouse, rat) and is validated for applications like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity for FADD makes it valuable in studying dysregulated cell death pathways in cancer, autoimmune diseases, and neurodegenerative disorders. Researchers often use Ab-191 to explore FADD's role in crosstalk between apoptosis and necroptosis, particularly in contexts where caspase-8 activity is compromised. Controls, such as FADD-knockout cell lines, are recommended to confirm signal specificity due to potential cross-reactivity with unrelated proteins in certain experimental conditions.
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