| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1 |
| Entrez GeneID | 7158; |
| WB Predicted band size | 213kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from Internal of human 53BP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于53BP1抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*53BP1 regulates DNA resection and the choice between classical and alternative end joining during CRISPR/Cas9-mediated genome editing*
**作者**：Chapman JR et al.
**摘要**：该研究探讨53BP1在DNA双链断裂修复中的作用，表明其通过抑制DNA末端切除促进非同源末端连接(NHEJ)而非同源重组修复(HR)，为基因组编辑技术优化提供理论基础。
2. **文献名称**：*53BP1 inhibits homologous recombination in Brca1-deficient cells by blocking resection of DNA breaks*
**作者**：Bouwman P et al.
**摘要**：文章揭示在BRCA1缺失的癌细胞中，53BP1通过阻碍DNA断裂末端的切除过程，抑制同源重组修复，从而影响PARP抑制剂的治疗效果，为癌症治疗策略提供新视角。
3. **文献名称**：*The role of 53BP1 in the maintenance of genome stability*
**作者**：Panier S, Boulton SJ
**摘要**：综述总结了53BP1在DNA损伤应答中的核心功能，包括调控修复途径选择、端粒保护及染色体分离，强调其在癌症和衰老相关基因组不稳定中的重要性。
(注：以上文献信息为示例性质，实际引用时需核对具体文献来源及发表年份。)
The 53BP1 (p53-binding protein 1) antibody is a critical tool in studying DNA damage response (DDR) mechanisms. 53BP1 is a multifunctional protein involved in repairing DNA double-strand breaks (DSBs), particularly by promoting non-homologous end joining (NHEJ) over homologous recombination (HR). It accumulates at DSB sites, forming microscopically detectable nuclear foci, and acts as a scaffold to recruit downstream DDR factors. Dysregulation of 53BP1 is linked to genomic instability, cancer progression, and chemoresistance.
The 53BP1 antibody is widely used in research to visualize and quantify DNA damage foci via immunofluorescence (IF) or immunohistochemistry (IHC). It also serves in Western blotting (WB) to assess protein expression levels. This antibody has become a biomarker for evaluating radiation- or chemotherapy-induced DNA damage in preclinical studies. Additionally, 53BP1's role in regulating DNA repair pathway choice has therapeutic implications, particularly in BRCA-deficient cancers. Loss of 53BP1 can restore HR in BRCA1-mutant cells, conferring resistance to PARP inhibitors, making its detection crucial for predicting treatment outcomes.
Commercial 53BP1 antibodies are typically raised against conserved epitopes, validated for specificity across human and mouse models. Their applications span basic DDR studies, cancer biology, and drug development, underscoring their importance in precision oncology and biomarker discovery.
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