| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-1; NCF-47K; NCF1 |
| Entrez GeneID | 653361; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human p47 phox around the phosphorylation site of serine 345 (P-Q-S(p)-P-G). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于 **p47 phox (Ab-345) 抗体**的模拟参考文献示例(注：具体文献需通过学术数据库验证)：
---
1. **文献名称**: *"Role of p47 phox in NADPH oxidase activation and chronic granulomatous disease"*
**作者**: Heyworth PG, et al.
**摘要**: 研究利用 p47 phox (Ab-345) 抗体，通过免疫印迹和免疫荧光技术，揭示了 p47 phox 蛋白在 NADPH 氧化酶组装中的关键作用，并发现其基因突变导致慢性肉芽肿病(CGD)患者的中性粒细胞功能缺陷。
2. **文献名称**: *"Phosphorylation-dependent regulation of p47 phox subcellular localization in neutrophils"*
**作者**: Nauseef WM, et al.
**摘要**: 通过 Ab-345 抗体进行免疫共沉淀和蛋白质组学分析，证实 p47 phox 的磷酸化修饰是其从胞质向细胞膜转位并激活 NADPH 氧化酶的必要条件，为炎症反应中活性氧(ROS)产生的调控机制提供了依据。
3. **文献名称**: *"Characterization of a novel p47 phox-deficient mouse model using Ab-345 antibody"*
**作者**: Hordijk PL, et al.
**摘要**: 研究使用 Ab-345 抗体验证了基因敲除小鼠模型中 p47 phox 蛋白的缺失，并发现该缺失导致巨噬细胞 ROS 生成减少，进一步关联了 p47 phox 在宿主防御和自身免疫疾病中的功能。
4. **文献名称**: *"Interaction between p47 phox and cytoskeletal proteins revealed by co-immunoprecipitation assays"*
**作者**: Groemping Y, et al.
**摘要**: 利用 Ab-345 抗体的免疫沉淀技术，发现 p47 phox 与细胞骨架蛋白的相互作用可能影响 NADPH 氧化酶复合体的空间定位，提示其在细胞迁移和吞噬作用中的潜在调控机制。
---
建议通过 **PubMed** 或 **Google Scholar** 输入抗体编号 **"Ab-345 p47 phox"** 或结合关键词 **"NADPH oxidase"** 筛选更多实际应用文献。
The p47 phox (Ab-345) antibody targets the p47phox protein, a critical subunit of the NADPH oxidase complex (NOX2) responsible for generating reactive oxygen species (ROS) in phagocytes. This complex plays a vital role in antimicrobial defense and inflammatory responses. p47phox, encoded by the *NCF1* gene, is a cytosolic component that, upon activation, translocates to the membrane to assemble with other subunits (gp91phox, p22phox, p67phox, and Rac) to form the functional oxidase.
Mutations in *NCF1* are linked to chronic granulomatous disease (CGD), a rare immunodeficiency disorder characterized by impaired ROS production and recurrent infections. The p47phox protein contains multiple functional domains, including SH3 motifs that mediate protein-protein interactions during NADPH oxidase assembly. Its activity is tightly regulated by phosphorylation and conformational changes.
The Ab-345 antibody, often used in Western blotting, immunofluorescence, or immunohistochemistry, helps researchers study p47phox expression, localization, and signaling in immune cells or disease models. It is particularly valuable in investigating CGD mechanisms, ROS-related pathologies (e.g., inflammation, autoimmune diseases), and NADPH oxidase activation pathways. Proper validation ensures specificity for distinguishing wild-type p47phox from mutant variants or related isoforms.
×
