| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCR4-associated factor 2; CCR4-NOT transcription complex subunit 2; CCR4-NOT transcription complex; subunit 2; CDC36 |
| Entrez GeneID | 4848; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human CNOT2 around the phosphorylation site of serine 101 (S-L-S(p)-Q-G). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CNOT2(可能涉及抗体Ab-101)的3篇参考文献示例(注：具体抗体编号可能因厂商而异，以下内容为模拟文献)：
1. **"CNOT2 regulates stem cell plasticity in murine embryonic stem cells"**
*作者：Smith A, et al. (2018)*
摘要：研究揭示了CNOT2通过CCR4-NOT复合物调控胚胎干细胞多能性和分化，利用CNOT2抗体(Ab-101)进行免疫沉淀，证实其与转录抑制因子相互作用，影响关键基因表达。
2. **"The role of CNOT2 in mRNA decay and cancer progression"**
*作者：Lee J, et al. (2020)*
摘要：通过Western blot和免疫组化(使用CNOT2抗体Ab-101)发现，CNOT2在结直肠癌中表达下调，其缺失导致促癌基因mRNA稳定性增加，加速肿瘤侵袭。
3. **"CNOT2 modulates antiviral immune response via post-transcriptional regulation"**
*作者：Chen H, et al. (2021)*
摘要：研究表明CNOT2通过降解病毒相关mRNA参与先天免疫应答，实验中使用CNOT2特异性抗体(Ab-101)敲低蛋白后，细胞抗病毒能力显著下降。
*注：以上文献为示例，实际文献需通过PubMed或抗体厂商(如Abcam、CST等)提供的引用列表查询具体编号Ab-101的用途。*
The CNOT2 (Ab-101) antibody is a tool designed to target the CNOT2 protein, a critical subunit of the CCR4-NOT complex—a conserved, multi-functional regulator of gene expression. The CCR4-NOT complex plays central roles in transcriptional and post-transcriptional regulation, including mRNA deadenylation, decay, and translational repression, thereby influencing diverse cellular processes such as development, differentiation, and stress responses. CNOT2 contributes to the structural integrity of the complex and mediates interactions with other proteins or RNA molecules, facilitating its role in mRNA metabolism and chromatin modification.
Research using CNOT2-specific antibodies like Ab-101 has been pivotal in elucidating the protein’s involvement in cellular homeostasis and disease. For example, studies link CNOT2 dysregulation to cancers, neurological disorders, and immune dysfunction, highlighting its potential as a therapeutic target. The Ab-101 antibody, typically validated for applications like Western blotting, immunoprecipitation, or immunofluorescence, enables researchers to probe CNOT2 expression, localization, and interactions in various models. Its specificity and reliability are crucial for dissecting the CCR4-NOT complex’s mechanisms, including its interplay with microRNAs, RNA-binding proteins, or signaling pathways. Overall, CNOT2 (Ab-101) serves as a key reagent for advancing understanding of post-transcriptional regulation and its implications in health and disease.
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