| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; kinase Mnk1; Map kinase interacting kinase; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1 |
| Entrez GeneID | 8569; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human Mnk1 around the phosphorylation site of threonine 385 (L-P-T(p)-P-Q). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 Mnk1 (Ab-385) 抗体的 3 篇文献示例(注：文献为模拟示例，实际引用需核实)：
1. **"Mnk1 regulates eIF4E phosphorylation and mTOR signaling in cancer progression"**
*作者：Zhang et al. (2015)*
摘要：研究通过使用 Mnk1 (Ab-385) 抗体，发现 Mnk1 通过磷酸化 eIF4E 增强肿瘤细胞蛋白质翻译，促进结直肠癌侵袭，为靶向 Mnk1-eIF4E 轴的癌症治疗提供依据。
2. **"Role of Mnk1 in T cell activation via MAPK pathway modulation"**
*作者：Lee & Thompson (2018)*
摘要：利用 Mnk1 (Ab-385) 进行免疫印迹分析，揭示 Mnk1 在 T 细胞受体激活后调控 IL-2 分泌的关键作用，表明其通过 p38 MAPK 通路影响免疫反应。
3. **"Mnk1 deficiency attenuates neuroinflammation in Alzheimer's disease models"**
*作者：Wang et al. (2020)*
摘要：通过 Mnk1 (Ab-385) 抗体检测小鼠脑组织，发现 Mnk1 缺失减少 Aβ 斑块相关炎症因子表达，提示其作为神经退行性疾病治疗靶点的潜力。
(注：以上文献信息为模拟生成，实际研究中建议通过 PubMed 或抗体供应商官网验证具体引用。)
**Background of Mnk1 (Ab-385) Antibody**
The Mnk1 (MAP kinase-interacting serine/threonine kinase 1) antibody, clone Ab-385. is a tool designed to detect and study Mnk1. a serine/threonine kinase involved in cellular signaling pathways. Mnk1 interacts with the mitogen-activated protein kinase (MAPK) pathway, particularly downstream of ERK and p38 kinases, and plays a role in phosphorylating eukaryotic initiation factor 4E (eIF4E), a critical regulator of mRNA translation. This phosphorylation event is implicated in modulating protein synthesis, cell proliferation, and stress responses.
The Ab-385 antibody is commonly used in applications like Western blotting, immunoprecipitation, and immunofluorescence to investigate Mnk1 expression, activation, and localization in various cell types and tissues. It specifically recognizes human, mouse, and rat Mnk1. aiding in research on its role in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. Studies using this antibody have contributed to understanding Mnk1's dual regulatory functions—both in promoting tumorigenesis via eIF4E-dependent oncogenic translation and in mediating stress adaptation through p38-MAPK signaling.
As Mnk1 is a potential therapeutic target, the Ab-385 antibody serves as a key reagent for validating Mnk1 inhibition in preclinical models and exploring its crosstalk with other signaling cascades. Its specificity and reliability make it valuable for dissecting Mnk1's contributions to cellular homeostasis and disease mechanisms.
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