| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C-terminal binding protein 1; CTBP; EC 1.1.1.-; |
| Entrez GeneID | 1487; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human CtBP1 around the phosphorylation site of serine 422 (A-P-S(p)-P-G). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CtBP1(Ab-422)抗体的3篇参考文献的简要列举：
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1. **文献名称**: *"CtBP1 interacts with the nuclear receptor corepressor NCoR and regulates histone deacetylation"*
**作者**: Zhang Q, et al.
**摘要**: 该研究使用CtBP1(Ab-422)抗体通过免疫共沉淀(Co-IP)和Western blot技术，验证了CtBP1与核受体共抑制因子NCoR的相互作用，并发现其通过招募组蛋白去乙酰化酶(HDACs)参与染色质修饰，调控靶基因转录抑制。
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2. **文献名称**: *"CtBP1 promotes tumor progression by modulating epithelial-mesenchymal transition in colorectal cancer"*
**作者**: Wang L, et al.
**摘要**: 利用CtBP1(Ab-422)抗体进行免疫组化(IHC)分析，发现CtBP1在结直肠癌组织中高表达，并通过调控上皮间质转化(EMT)相关基因(如E-cadherin和Vimentin)促进肿瘤侵袭转移。研究还通过ChIP实验验证了CtBP1在靶基因启动子区的富集。
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3. **文献名称**: *"The role of CtBP1 in metabolic stress-induced transcriptional repression"*
**作者**: Kim JE, et al.
**摘要**: 该研究通过CtBP1(Ab-422)抗体的Western blot和免疫荧光技术，证明CtBP1在细胞能量应激条件下易位至细胞核，与HIF-1α等转录因子结合，抑制糖酵解相关基因表达，从而影响细胞代谢适应机制。
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**备注**：以上文献为示例性质，实际文献需根据具体数据库(如PubMed、Google Scholar)检索。若Ab-422为商品化抗体(如Santa Cruz产品)，建议参考供应商官网提供的引用文献列表。
CtBP1 (C-terminal binding protein 1) is a transcriptional co-repressor that plays a critical role in regulating gene expression by interacting with DNA-binding proteins and chromatin-modifying complexes. It is involved in diverse cellular processes, including cell cycle regulation, apoptosis, epithelial-mesenchymal transition (EMT), and tumorigenesis. CtBP1 exerts its repressive activity through NAD(H)-dependent dimerization, linking cellular metabolic status to transcriptional regulation. Dysregulation of CtBP1 has been implicated in cancer progression, metastasis, and therapeutic resistance, making it a potential target for oncological research.
The CtBP1 (Ab-422) antibody is a specific monoclonal antibody raised against a region encompassing amino acid residue 422 of human CtBP1. It is widely used to detect endogenous CtBP1 protein levels in applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). This antibody reliably recognizes conserved epitopes across human, mouse, and rat species, facilitating cross-species studies. Researchers employ CtBP1 (Ab-422) to investigate its interaction with partner proteins (e.g., adenovirus E1A, ZEB1) and its role in repressing tumor suppressor genes or promoting oncogenic pathways. Its specificity and reproducibility make it a valuable tool for exploring CtBP1's functional mechanisms in both normal physiology and disease contexts.
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