| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Collapsin response mediator protein 2; CRMP2; Dihydropyrimidinase related protein-2; DPYL2; DPYSL2 |
| Entrez GeneID | 1808; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human CRMP-2 around the phosphorylation site of threonine 509 (S-V-T(p)-P-K). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CRMP-2(使用Ab-509抗体)的示例性参考文献(内容为模拟，仅供参考)：
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1. **文献名称**: *Phosphorylation of CRMP-2 by GSK-3β regulates axonal growth in Alzheimer's disease models*
**作者**: Smith A, et al.
**摘要**: 本研究利用CRMP-2 (Ab-509)抗体，通过Western blot和免疫组化分析发现，GSK-3β介导的CRMP-2磷酸化在阿尔茨海默病小鼠模型中显著增加，导致轴突导向缺陷。抑制该磷酸化可改善神经元功能。
2. **文献名称**: *CRMP-2 modulates microtubule dynamics in spinal cord injury recovery*
**作者**: Lee J, et al.
**摘要**: 使用CRMP-2 (Ab-509)抗体检测大鼠脊髓损伤后CRMP-2的表达变化。结果显示，损伤后CRMP-2降解增多，过表达CRMP-2可促进轴突再生，提示其作为神经修复靶点的潜力。
3. **文献名称**: *Differential roles of CRMP-2 isoforms in neurodevelopmental disorders*
**作者**: Chen L, et al.
**摘要**: 通过CRMP-2 (Ab-509)抗体分选不同剪接变体，发现CRMP-2B在自闭症模型小鼠前额叶皮层中异常表达，可能通过影响突触可塑性参与疾病发生。
4. **文献名称**: *CRMP-2 interaction with tubulin: Implications for chemotherapeutic-induced neuropathy*
**作者**: Gupta S, et al.
**摘要**: 采用CRMP-2 (Ab-509)进行免疫共沉淀实验，揭示化疗药物通过破坏CRMP-2与微管蛋白的结合诱发周围神经病变，靶向此通路或可减轻副作用。
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**说明**：以上文献为示例，实际引用需根据具体研究检索PubMed或Google Scholar。若需真实文献，建议使用关键词“CRMP-2 antibody Ab-509”或“CRMP-2 phosphorylation”在学术数据库中查询。
CRMP-2 (Collapsin Response Mediator Protein-2), also known as DPYSL2. is a cytosolic phosphoprotein primarily expressed in the nervous system. It plays a critical role in neuronal development, axon guidance, and synaptic plasticity by regulating microtubule dynamics and cytoskeletal organization. CRMP-2 interacts with tubulin heterodimers to promote microtubule assembly, influencing neurite outgrowth and neuronal polarization. Dysregulation of CRMP-2 has been implicated in neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), neuropathic pain, and cancer metastasis. Phosphorylation by kinases like GSK-3β and Cdk5 modulates its activity, with hyperphosphorylated forms being associated with pathological aggregates in Alzheimer’s brains.
The CRMP-2 (Ab-509) antibody is a monoclonal antibody specifically targeting epitopes within the protein, often used in research to detect CRMP-2 expression, post-translational modifications, or localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies employ Ab-509 to explore CRMP-2’s role in neurological disorders or cancer progression, where its overexpression or phosphorylation status may serve as a biomarker. Notably, CRMP-2 has emerged as a potential therapeutic target, with inhibitors under investigation for neuroprotection or anti-metastatic effects. The antibody’s specificity makes it a valuable tool for elucidating CRMP-2’s functional mechanisms in health and disease.
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