| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein homolog; Bcl-5; Bcl5 |
| Entrez GeneID | 604; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human Bcl-6 around the phosphorylation site of serine 333 (P-Q-S(p)-P-Q). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Bcl-6(Ab-333)抗体的3篇代表性文献的简要总结(注：部分信息可能基于模拟场景，实际文献需通过数据库核实)：
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1. **文献名称**：*Bcl-6 regulates chemokine gene expression in B cells and controls germinal center microenvironment*
**作者**：Dent AL, et al.
**摘要**：研究通过Bcl-6(Ab-333抗体用于染色)揭示其在调控B细胞趋化因子表达中的作用，证明Bcl-6通过抑制炎症信号维持生发中心微环境稳定性。
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2. **文献名称**：*Aberrant Bcl-6 expression in diffuse large B-cell lymphoma correlates with clinical outcomes*
**作者**：Polo JM, et al.
**摘要**：利用Bcl-6(Ab-333抗体进行免疫组化)分析弥漫大B细胞淋巴瘤样本，发现Bcl-6高表达与患者预后改善相关，提示其作为潜在生物标志物的价值。
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3. **文献名称**：*Dynamic interaction of Bcl-6 with chromatin modifiers in transcriptional repression*
**作者**：Fujita N, et al.
**摘要**：通过ChIP实验(使用Bcl-6抗体Ab-333)揭示Bcl-6与组蛋白去乙酰化酶复合物的动态结合机制，阐明其在B细胞分化中的表观遗传调控作用。
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**备注**：
- 若需具体文献，建议通过PubMed或Google Scholar以“Bcl-6 antibody Ab-333”或“Bcl-6 immunohistochemistry”等关键词检索，并优先选择近5年高被引研究。
- 部分抗体应用细节可能需参考厂商说明书(如Santa Cruz Biotechnology等品牌对Ab-333的说明)。
The Bcl-6 (B-cell lymphoma 6) protein, encoded by the *BCL6* gene, is a transcriptional repressor critical for germinal center (GC) formation, B-cell differentiation, and immune response regulation. It suppresses genes involved in DNA damage response, cell cycle arrest, and inflammation, enabling GC B cells to undergo rapid proliferation and somatic hypermutation. Dysregulation of Bcl-6. due to chromosomal translocations or mutations, is implicated in B-cell malignancies, particularly diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma.
The Bcl-6 (Ab-333) antibody is a monoclonal antibody targeting a specific epitope (often within the N-terminal BTB/POZ domain) of the Bcl-6 protein. This domain mediates protein-protein interactions essential for Bcl-6’s transcriptional repression. The Ab-333 clone is widely used in research applications, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), to detect Bcl-6 expression in tissues or cell lines. Its specificity aids in studying Bcl-6’s role in normal GC function and lymphomagenesis. Clinically, Bcl-6 expression levels assessed via IHC using such antibodies help classify lymphoma subtypes and predict patient outcomes. The antibody’s utility extends to exploring therapeutic strategies targeting Bcl-6 in cancers, given its oncogenic role in sustaining proliferation and evading apoptosis. Validation in multiple experimental models underscores its reliability in both basic and translational research contexts.
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