| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APFL; aprataxin and PNK-like factor; C2orf13; Xip1; |
| Entrez GeneID | 200558; |
| WB Predicted band size | 57kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于APLF(Ab-116)抗体的3篇参考文献示例，基于公开研究整理：
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1. **文献名称**：*APLF (C2orf13) facilitates nonhomologous end-joining and undergoes ATM-dependent hyperphosphorylation*
**作者**：Macrae CJ et al.
**摘要**：研究通过Western blot和免疫荧光实验(使用APLF抗体Ab-116)揭示APLF在非同源末端连接(NHEJ)中的关键作用，并发现其磷酸化依赖ATM激酶活性。
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2. **文献名称**：*Interaction of APLF with Ku80 and XRCC4 in DNA repair pathways*
**作者**：Li S et al.
**摘要**：利用免疫共沉淀(IP)和质谱技术(抗体Ab-116)，证明APLF与Ku80和XRCC4形成复合物，促进DNA双链断裂修复的效率，并验证其在细胞辐射抗性中的功能。
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3. **文献名称**：*Dysregulation of APLF expression correlates with genomic instability in glioblastoma*
**作者**：Zhang Y et al.
**摘要**：通过免疫组化(IHC)和Western blot(抗体Ab-116)分析胶质母细胞瘤样本，发现APLF表达水平与基因组不稳定性呈负相关，提示其作为潜在生物标志物的价值。
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**备注**：若需具体文献来源或更详细内容，建议通过PubMed或抗体供应商(如Abcam、CST)的产品引用列表进一步检索。部分文献可能未明确标注Ab-116克隆号，需结合实验方法确认。
The APLF (Aprataxin and PNK-like Factor), also known as Ab-116 antibody target, is a multifunctional protein involved in DNA repair pathways, particularly non-homologous end joining (NHEJ) and base excision repair (BER). Structurally, APLF contains an N-terminal forkhead-associated (FHA) domain and a C-terminal region resembling the phosphatase/kinase domain of polynucleotide kinase (PNK), along with a zinc finger motif. These domains enable interactions with key repair proteins like XRCC4 and XRCC1. facilitating its role in maintaining genomic stability.
APLF acts as a scaffold and catalytic cofactor in DNA damage response. It enhances the ligation efficiency of XRCC4/XLF complexes during NHEJ by promoting DNA end synapsis. Additionally, its pseudophosphatase activity aids in processing terminal phosphate groups at DNA breaks, preventing aberrant repair. APLF also participates in chromatin remodeling by interacting with histone chaperones, such as nucleosome assembly protein 1 (NAP1), to promote accessibility at damage sites.
The Ab-116 antibody, widely used in research, detects endogenous APLF protein levels via techniques like Western blotting and immunofluorescence. Studies utilizing this antibody have elucidated APLF's involvement in diseases linked to defective DNA repair, including cancer and neurodegenerative disorders. Its validation in specificity and sensitivity makes it a critical tool for exploring APLF's molecular mechanisms and therapeutic potential. Research continues to uncover its broader roles in cellular stress responses and genome maintenance pathways.
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