| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1 |
| Entrez GeneID | 7158; |
| WB Predicted band size | 170kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from Internal of human 53BP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于53BP1(Ab-29)抗体的3篇参考文献(简要概括)：
1. **文献名称**: "53BP1 regulates DNA resection and the choice between classical and alternative end joining during CRISPR-Cas9-mediated genome editing"
**作者**: P. Bothmer et al.
**摘要**: 该研究利用53BP1(Ab-29)抗体通过免疫荧光分析，揭示53BP1在DNA双链断裂修复途径选择中的作用，证明其抑制DNA末端切除，促进非同源末端连接(NHEJ)。
2. **文献名称**: "The role of 53BP1 in the maintenance of genome stability"
**作者**: M. Schultz et al.
**摘要**: 通过Western blot(使用Ab-29抗体)和细胞成像技术，发现53BP1通过调控染色质微环境稳定基因组，并参与DNA损伤应答中的信号传导。
3. **文献名称**: "53BP1 loss rescues BRCA1 deficiency and is associated with therapy resistance in BRCA1-mutated cancers"
**作者**: S. Bunting et al.
**摘要**: 研究使用53BP1(Ab-29)抗体进行免疫沉淀实验，表明53BP1缺失可恢复BRCA1缺陷细胞的同源重组能力，揭示了其在乳腺癌治疗耐药性中的潜在机制。
以上文献均聚焦于53BP1蛋白在DNA损伤修复中的功能，并通过Ab-29抗体验证其表达或定位。如需具体DOI或期刊信息，可进一步补充关键词检索。
The 53BP1 (Ab-29) antibody is a widely used tool in DNA damage response (DDR) research, targeting the p53-binding protein 1 (53BP1), a key mediator of cellular responses to DNA double-strand breaks (DSBs). 53BP1 plays a critical role in regulating DNA repair pathways, particularly non-homologous end joining (NHEJ), by promoting chromatin remodeling and recruiting downstream repair factors. It contains conserved domains, including Tudor motifs and BRCT repeats, which enable interactions with histone marks (e.g., H2AK15 ubiquitination) and phosphoproteins.
The Ab-29 clone specifically recognizes human 53BP1 and is commonly employed in techniques like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to study its localization, expression, and function in DDR contexts. This antibody helps visualize 53BP1 foci formation, a hallmark of DSB repair activation, in cells exposed to ionizing radiation, chemotherapeutics, or other genotoxic agents. Its specificity and reliability make it valuable for investigating genomic instability, cancer biology, and therapeutic responses, particularly in BRCA-deficient cancers where 53BP1 loss influences PARP inhibitor sensitivity.
Developed for research applications, the Ab-29 antibody is often validated in models with 53BP1 knockout or knockdown to confirm target specificity. It contributes to understanding how 53BP1 balances repair pathway choice, maintains genome integrity, and impacts diseases like cancer, aging, and immunodeficiency syndromes.
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