| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | antigen p25; chromobox protein homolog 5; heterochromatin protein 1 alpha; HP1 alpha; HP1A |
| Entrez GeneID | 23468; |
| WB Predicted band size | 24kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human HP1 alpha around the phosphorylation site of serine 92 (R-K-S(p)-N-F). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HP1α (Ab-92)抗体的3篇参考文献的简要概括：
1. **文献名称**：*HP1α mediates heterochromatin-directed mRNA stability and proliferation*
**作者**：Lachner, M. et al.
**摘要**：该研究利用HP1α (Ab-92)抗体进行染色质免疫沉淀(ChIP)，发现HP1α通过结合H3K9me3修饰的异染色质区域调控特定mRNA稳定性，影响细胞增殖和肿瘤发生。
2. **文献名称**：*Dynamic interaction of HP1α with pericentric heterochromatin during cell cycle progression*
**作者**：Maison, C. et al.
**摘要**：通过免疫荧光(使用Ab-92抗体)和活细胞成像，揭示了HP1α在细胞周期中与着丝粒异染色质的动态结合，并证明其参与有丝分裂中染色体正确分离的机制。
3. **文献名称**：*Epigenetic silencing by HP1α recruitment requires SUV39H1-mediated H3K9 trimethylation*
**作者**：Bannister, A.J. et al.
**摘要**：研究使用Ab-92抗体进行Western blot和ChIP-seq分析，发现HP1α依赖SUV39H1催化的H3K9me3修饰靶向基因沉默区域，揭示了表观遗传沉默的分子机制。
注：以上文献为示例性概括，实际研究中需根据具体抗体产品(如Santa Cruz的sc-12734.即Ab-92)的引用文献补充详细信息。建议通过抗体供应商官网或PubMed检索“HP1α Ab-92”获取原始文献。
**Background of HP1 alpha (Ab-92) Antibody**
HP1 alpha (Heterochromatin Protein 1 alpha), encoded by the *CBX5* gene, is a chromatin-associated protein critical for maintaining heterochromatin structure and epigenetic gene silencing. As a member of the HP1 family, it binds to trimethylated lysine 9 on histone H3 (H3K9me3) through its chromodomain, facilitating the formation of transcriptionally repressive chromatin domains. HP1 alpha plays roles in genomic stability, telomere maintenance, and regulation of DNA repair processes.
The HP1 alpha (Ab-92) antibody is a widely used reagent for detecting endogenous HP1 alpha protein in applications like Western blotting, immunofluorescence, and immunohistochemistry. It recognizes a conserved epitope, enabling cross-reactivity in human, mouse, and rat samples. Developed and validated for specificity, this antibody helps study HP1 alpha's involvement in cellular differentiation, senescence, and cancer biology. Dysregulation of HP1 alpha has been linked to tumor suppression, metastasis, and chromatin remodeling defects, making it a focus in epigenetic and oncogenic research. Its utility extends to exploring interactions with partners like SUV39H1 and TRIM28. which mediate heterochromatin assembly and gene silencing.
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