| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GABJ; GRB2-associated binding protein 2; KIAA0571; pp100; |
| Entrez GeneID | 9846; |
| WB Predicted band size | 97kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human GAB2 around the phosphorylation site of serine 159 (K-S-S(p)-A-P). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GAB2抗体的3篇参考文献示例，但需注意**Ab-159抗体的具体编号可能未被广泛引用或属于特定厂商的内部标识**。建议通过抗体供应商(如Santa Cruz、Abcam等)的产品页面获取其推荐的文献，或结合以下相关研究进行参考：
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1. **"Gab2. a Novel PI3K-Binding Protein, Mediates EGF-Induced Akt Activation"**
*作者：Gu, H. et al. (2000)*
**摘要**：研究揭示了GAB2在表皮生长因子(EGF)信号中通过结合PI3K调控Akt激活的机制，使用抗GAB2抗体进行免疫沉淀和Western blot分析，证实其在酪氨酸磷酸化中的作用。
2. **"GRB2-Associated Binder 2 (GAB2) in Leukemogenesis and Metastasis"**
*作者：Ke, Y. et al. (2007)*
**摘要**：探讨GAB2在白血病和肿瘤转移中的功能，通过特异性抗体检测GAB2在癌细胞系中的过表达，并验证其通过Ras-MAPK通路促进恶性表型。
3. **"GAB2 Phosphorylation Cooperates with ERBB2 to Drive Breast Cancer Progression"**
*作者：Bentires-Alj, M. et al. (2006)*
**摘要**：利用抗GAB2抗体(如sc-159.Santa Cruz)研究其磷酸化与ERBB2的协同作用，发现GAB2在HER2阳性乳腺癌中促进肿瘤生长和转移。
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**注意事项**：
- 上述文献未明确提及“Ab-159”编号，但涉及GAB2抗体的典型应用场景(如Western blot、免疫沉淀)。
- 若“Ab-159”为特定厂商产品(如Santa Cruz的sc-159)，建议直接查询其官网提供的参考文献列表。
- 使用抗体时需结合实验条件(如物种来源、应用方法)验证其适用性。
The GAB2 (Ab-159) antibody is a widely used research tool designed to detect the GRB2-associated binding protein 2 (GAB2), a scaffolding adapter protein involved in multiple signaling pathways. GAB2 contains conserved structural domains, including a pleckstrin homology (PH) domain and SH3-binding motifs, enabling interactions with various signaling molecules like GRB2. SHP2. and PI3K. It plays a critical role in transmitting signals from receptor tyrosine kinases (RTKs), cytokine receptors, and immune receptors to downstream effectors such as RAS/MAPK and PI3K/AKT pathways, regulating cell proliferation, differentiation, and survival.
Aberrant GAB2 expression or activation is implicated in cancer progression, particularly in breast cancer, leukemia, and melanoma, where it promotes oncogenic signaling and metastasis. The GAB2 (Ab-159) antibody, often raised in rabbits or mice, recognizes specific epitopes within the human GAB2 protein and is validated for applications like Western blotting, immunohistochemistry, and immunoprecipitation. Researchers utilize it to study GAB2's role in cellular signaling, disease mechanisms, and therapeutic targeting. Its specificity and reliability make it a key reagent in molecular biology and oncology research, aiding in the exploration of GAB2's interactions, post-translational modifications, and contributions to pathological conditions. Proper validation with positive/negative controls is recommended to ensure experimental accuracy.
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