| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 1.14.13.39; HEP-NOS; hepatocyte NOS; inducible NOS; nitric oxide synthase |
| Entrez GeneID | 4843; |
| WB Predicted band size | 131kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human iNOS around the phosphorylation site of tyrosine 151 (Q-Y-Y(p)-G-S). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **iNOS (Ab-151) 抗体**的模拟参考文献示例(仅供参考，非真实文献)：
1. **"iNOS Expression in Macrophage-Mediated Inflammation"**
- 作者：Smith A, et al.
- 摘要：研究通过Western blot和免疫组化分析，使用iNOS (Ab-151)抗体检测脂多糖(LPS)诱导的巨噬细胞中iNOS表达上调，证实其在炎症反应中的关键作用。
2. **"Role of iNOS in Sepsis-Induced Organ Injury"**
- 作者：Lee C, et al.
- 摘要：在小鼠脓毒症模型中，利用iNOS (Ab-151)抗体进行组织染色，发现肾脏和肝脏中iNOS表达显著增加，提示其参与多器官功能障碍的病理机制。
3. **"iNOS Inhibition Attenuates Atherosclerotic Plaque Formation"**
- 作者：Garcia R, et al.
- 摘要：通过iNOS (Ab-151)抗体检测动脉粥样硬化斑块中的酶活性，结合药理学抑制实验，证明iNOS促进斑块内炎症细胞浸润和氧化应激。
4. **"iNOS in Tumor-Associated Macrophages Promotes Cancer Progression"**
- 作者：Wang Y, et al.
- 摘要：利用iNOS (Ab-151)抗体分析肿瘤微环境中巨噬细胞的表型，发现iNOS高表达与肿瘤血管生成和免疫抑制相关，提示其作为治疗靶点的潜力。
**注**：以上文献为示例性内容，实际研究中需通过数据库(如PubMed、Web of Science)以“iNOS Ab-151”为关键词检索真实引用。部分抗体生产商(如Santa Cruz Biotechnology)官网也可能提供相关参考文献列表。
The inducible nitric oxide synthase (iNOS), also known as NOS2. is a key enzyme that catalyzes the production of nitric oxide (NO) from L-arginine. It is expressed in various immune cells (e.g., macrophages) and tissues under inflammatory or pathogenic stimuli, playing critical roles in antimicrobial defense, immune regulation, and pathological processes like chronic inflammation. Unlike constitutive NOS isoforms, iNOS is transcriptionally induced by cytokines (e.g., TNF-α, IFN-γ) or microbial components, leading to sustained NO release. Dysregulation of iNOS is linked to sepsis, neurodegenerative diseases, and cancer progression.
The iNOS (Ab-151) antibody is a monoclonal antibody specifically designed to detect the iNOS protein in research applications. It recognizes an epitope within the N-terminal region of human iNOS and has been validated for use in Western blotting, immunofluorescence, and immunohistochemistry. The antibody demonstrates high specificity, with minimal cross-reactivity to other NOS family members (e.g., eNOS, nNOS). Its utility extends to studying iNOS expression patterns in inflammatory models, infection responses, and cancer microenvironments. Proper validation using iNOS knockout controls or siRNA-mediated knockdown is recommended to confirm signal specificity. Researchers often employ this antibody to explore iNOS-mediated pathways in diseases like rheumatoid arthritis, atherosclerosis, and neurodegenerative disorders, aiding in mechanistic and therapeutic investigations. Storage at -20°C and adherence to dilution protocols are advised for optimal performance.
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