| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CD63 antigen; CD63 |
| Entrez GeneID | 12512 |
| WB Predicted band size | Calculated MW: 26 kDa; Observed MW: 40-70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of mouse CD63 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Androgen Receptor (Ab-363)抗体的3篇参考文献示例(注：部分信息为假设性示例，具体文献需根据实际数据库检索验证)：
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1. **文献名称**：*Androgen Receptor Phosphorylation and Activity are Regulated by Casein Kinase 2*
**作者**：Li Y, et al. (2015)
**摘要**：该研究利用Androgen Receptor (Ab-363)抗体进行免疫沉淀和Western blot分析，发现酪蛋白激酶2(CK2)通过磷酸化AR的Ser-363位点增强其转录活性，揭示了前列腺癌细胞中AR信号通路调控的新机制。
2. **文献名称**：*Subcellular Localization of Androgen Receptor in Prostate Cancer Metastasis*
**作者**：Smith J, et al. (2018)
**摘要**：通过免疫组织化学(IHC)和免疫荧光技术，使用Ab-363抗体检测AR蛋白在转移性前列腺癌组织中的核质分布，发现AR核定位与患者预后不良显著相关。
3. **文献名称**：*Development and Validation of a Novel Androgen Receptor-Specific Monoclonal Antibody for Functional Studies*
**作者**：Chen X, et al. (2020)
**摘要**：该研究报道了Ab-363抗体的开发与验证，证明其高特异性识别AR的磷酸化修饰形式，并成功应用于染色质免疫沉淀(ChIP)和流式细胞术，为AR功能研究提供了新工具。
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**提示**：实际检索时，建议通过PubMed或抗体供应商(如CST、Abcam)官网的“引用文献”板块，输入抗体编号(如Ab-363或Catalog#)获取准确文献。部分抗体编号可能对应厂商内部标识，需结合具体品牌确认。
The Androgen Receptor (AR) is a ligand-activated nuclear transcription factor belonging to the steroid hormone receptor superfamily. It mediates the biological effects of androgens, such as testosterone and dihydrotestosterone (DHT), by regulating gene expression critical for male sexual development, prostate function, and other androgen-dependent physiological processes. Structurally, AR comprises an N-terminal domain (NTD), a central DNA-binding domain (DBD), a hinge region, and a C-terminal ligand-binding domain (LBD). Post-translational modifications, including phosphorylation, modulate its activity, stability, and interactions with co-regulators.
The AR (Ab-363) antibody specifically recognizes AR phosphorylated at serine 363 (Ser363), a key regulatory site implicated in receptor activation, nuclear translocation, and transcriptional activity. Phosphorylation at Ser363 has been linked to AR signaling in prostate cancer progression, particularly in castration-resistant prostate cancer (CRPC), where AR remains active despite androgen deprivation therapies. This antibody is widely used in research to investigate AR signaling dynamics, hormone resistance mechanisms, and cross-talk with other pathways (e.g., EGFR or MAPK). Applications include Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) in human, mouse, and rat samples. By detecting this post-translational modification, AR (Ab-363) serves as a valuable tool for studying AR-driven oncogenesis and evaluating therapeutic strategies targeting AR or its regulatory kinases. Its specificity and reliability make it essential for both basic research and preclinical studies in endocrinology and oncology.
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