Rabbit Polyclonal STK39(Ab-325) Antibody

The STK39 (Ab-325) antibody is a tool for detecting serine/threonine kinase 39 (STK39), also known as SPAK (STE20/SPS1-related proline-alanine-rich kinase). STK39 is a key regulator of ion transport pathways, primarily through phosphorylation of cation-chloride cotransporters (e.g., NKCC1. NCC) in response to osmotic stress or intracellular signaling. It plays roles in blood pressure regulation, neuronal excitability, and cellular ion homeostasis. The Ab-325 antibody, often raised in rabbits or mice as a monoclonal or polyclonal reagent, typically targets specific epitopes within the kinase domain or regulatory regions of human, mouse, or rat STK39. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study STK39 expression, localization, and activation in tissues such as kidney, brain, and vascular endothelium. Research involving this antibody has contributed to understanding STK39's involvement in hypertension, neurological disorders, and electrolyte balance. Validation often includes knockout controls or peptide blocking to confirm specificity. Its applications extend to exploring STK39’s interaction with upstream kinases (e.g., WNKs) and downstream effectors in stress-responsive signaling cascades.