| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; kinase LIMK2; LIM domain kinase 2; LIMK-2; |
| Entrez GeneID | 3985; |
| WB Predicted band size | 72kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human LIMK2 around the phosphorylation site of serine 283 (R-R-S(p)-L-R). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LIMK2 (Ab-283)抗体的简要文献信息(注：因抗体编号可能对应特定厂商或研究，部分文献可能为假设性示例)：
1. **文献名称**：LIMK2 regulates tumor cell invasion via phosphorylation of cofilin in breast cancer
**作者**：Smith A, et al.
**摘要**：研究通过Western blot和免疫组化(使用LIMK2 Ab-283抗体)发现，LIMK2在乳腺癌中高表达，并通过磷酸化cofilin促进细胞骨架重塑，增强肿瘤侵袭能力。
2. **文献名称**：Role of LIMK2 in neuronal development: Insights from knockout models
**作者**：Chen L, et al.
**摘要**：利用LIMK2 Ab-283抗体进行免疫荧光染色，发现LIMK2缺失导致神经元突触结构异常，表明其在神经发育中通过调节actin动力学发挥作用。
3. **文献名称**：LIMK2/Ab-283 antibody validation in prostate cancer progression
**作者**：Wang Y, et al.
**摘要**：该研究验证了LIMK2 Ab-283抗体的特异性，并用于检测前列腺癌组织中LIMK2的表达水平，发现其与转移风险呈正相关。
4. **文献名称**：LIMK2-mediated signaling in TGF-β-induced fibrosis
**作者**：Kim J, et al.
**摘要**：通过免疫沉淀(使用LIMK2 Ab-283抗体)揭示LIMK2在TGF-β信号通路中活化，促进成纤维细胞向肌成纤维细胞转化，推动纤维化进程。
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**说明**：以上文献为基于LIMK2功能研究的典型方向模拟，实际文献需通过PubMed/Google Scholar以关键词“LIMK2 antibody Ab-283”或抗体编号检索确认。若该抗体为商业产品，可参考厂商官网(如CST/Abcam)提供的引用文献列表。
The LIMK2 (Ab-283) antibody is a research tool designed to detect LIM domain kinase 2 (LIMK2), a serine/threonine kinase involved in regulating cytoskeletal dynamics. LIMK2 plays a critical role in actin polymerization by phosphorylating and inactivating cofilin, an actin-depolymerizing protein. This kinase is activated by upstream Rho GTPase signaling pathways, particularly through ROCK and PAK kinases, and is implicated in cellular processes such as cell migration, division, and synaptic plasticity. Dysregulation of LIMK2 has been associated with cancer metastasis, neurological disorders, and cardiovascular diseases, making it a target for therapeutic research.
The Ab-283 antibody, typically a rabbit polyclonal antibody, recognizes endogenous LIMK2 protein across human, mouse, and rat samples. It is commonly used in applications like Western blotting, immunohistochemistry, and immunofluorescence to study LIMK2 expression and localization. The antibody targets a specific region near the C-terminus (around amino acid 283) of LIMK2. helping researchers distinguish it from its closely related isoform, LIMK1. Validation often includes testing in knockout cell lines or siRNA-mediated knockdown to confirm specificity. As LIMK2’s role in disease pathways gains attention, this antibody serves as a key reagent for exploring its mechanistic contributions and potential as a biomarker or drug target.
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