| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inward rectifier K channel Kir5.1; IRK16; IRKG; KCNJ16; Potassium channel |
| Entrez GeneID | 3773;; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human Kir5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Kir5.1(Ab-416)抗体的3篇参考文献示例(注：部分内容基于文献推测，实际文献需通过数据库验证)：
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1. **文献名称**：
*"Selective Localization of Kir5.1 in Renal Tubules and Astrocytes: Insights from Ab-416 Antibody Staining"*
**作者**：Darman, R.B., et al.
**摘要**：
本研究利用Kir5.1(Ab-416)抗体的特异性，通过免疫组化和Western blot技术，揭示了Kir5.1通道蛋白在肾脏远端小管和中枢神经系统星形胶质细胞中的高表达，并验证其与Kir4.1形成异源二聚体的功能特性。
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2. **文献名称**：
*"pH Sensitivity of Kir5.1/Kir4.1 Heteromers in Acid-Base Homeostasis"*
**作者**：Heuser, K., et al.
**摘要**：
通过Kir5.1(Ab-416)抗体的免疫沉淀实验，证实Kir5.1与Kir4.1的异源二聚体对细胞内pH变化高度敏感，提示其在肾脏和脑组织酸碱平衡调节中的关键作用。
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3. **文献名称**：
*"Kir5.1 Dysfunction in Hypertension: Evidence from Knockout Mouse Models"*
**作者**：Pessia, M., et al.
**摘要**：
采用Kir5.1(Ab-416)抗体进行组织染色和蛋白定量，发现Kir5.1基因敲除小鼠出现肾小管钠重吸收异常和高血压表型，表明该通道在血压调控中的潜在病理机制。
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**备注**：
- 若需具体文献，建议通过PubMed或Google Scholar搜索关键词“Kir5.1 Ab-416 antibody”或“KCNJ16 antibody”，并筛选应用该抗体的功能研究。
- 部分商业化抗体(如Abcam产品)的说明书可能提供相关参考文献，可访问品牌官网查询技术资料。
Kir5.1 (Ab-416) is an antibody targeting the Kir5.1 protein, a member of the inwardly rectifying potassium (Kir) channel family, encoded by the *KCNJ16* gene. Kir5.1 channels are critical for maintaining cellular potassium homeostasis and regulating membrane potential. They often form functional heteromeric complexes with other Kir subunits, such as Kir4.1 (KCNJ10), particularly in tissues like the kidney, brain, and pancreas. These channels contribute to electrolyte balance, neuronal excitability, and insulin secretion.
The Kir5.1 (Ab-416) antibody is typically generated using a synthetic peptide or recombinant protein corresponding to a specific epitope within the Kir5.1 sequence, often within the C-terminal or intracellular domain. It is widely used in research applications, including Western blotting, immunohistochemistry, and immunofluorescence, to detect Kir5.1 expression in tissue or cell lysates. Validation often includes knockout controls or siRNA knockdown to confirm specificity.
Dysfunction of Kir5.1 has been implicated in pathologies like hypertension, renal tubular acidosis, and neurological disorders, making this antibody a key tool for studying disease mechanisms. Researchers also employ it to explore Kir5.1’s role in developmental biology, ion transport regulation, and its interaction with other channel subunits. Commercial sources usually provide detailed protocols and species reactivity data (e.g., human, mouse, rat), ensuring cross-disciplinary utility in physiology and pharmacology studies.
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