| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CD104 antigen; GP150; ITB4; integrin beta-4; integrin beta-4C |
| Entrez GeneID | 3691; |
| WB Predicted band size | 202kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human ITGB4 around the phosphorylation site of tyrosine 1510 (R-D-Y(p)-S-T). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ITGB4(Ab-1510)抗体的参考文献示例(注：具体文献需通过学术数据库验证，以下为模拟结构供参考)：
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1. **"Integrin β4 signaling promotes tumor metastasis via EGFR-dependent pathways in lung adenocarcinoma"**
*Authors: Lee C, et al.*
**摘要**：本研究探讨了ITGB4在肺癌转移中的作用，利用ITGB4(Ab-1510)抗体进行免疫沉淀和Western blot分析，发现ITGB4通过EGFR信号通路增强肿瘤细胞侵袭能力。
2. **"Role of integrin β4 in epithelial-mesenchymal transition and drug resistance in breast cancer"**
*Authors: Zhang Y, et al.*
**摘要**：通过免疫组化(使用Ab-1510抗体)和基因沉默实验，证明ITGB4高表达与乳腺癌EMT表型及化疗耐药性显著相关。
3. **"Characterization of integrin β4 phosphorylation in keratinocyte adhesion"**
*Authors: Margadant C, et al.*
**摘要**：利用ITGB4(Ab-1510)进行免疫荧光染色，揭示了β4亚基磷酸化对上皮细胞黏着斑形成的调控机制，为皮肤屏障功能研究提供依据。
4. **"ITGB4 as a prognostic biomarker in pancreatic ductal adenocarcinoma"**
*Authors: Wang H, et al.*
**摘要**：通过组织微阵列结合Ab-1510抗体的免疫组化分析，证实ITGB4高表达与胰腺癌患者预后不良及淋巴转移相关。
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**建议**：
- 实际文献可通过 **PubMed/Google Scholar** 搜索关键词：`ITGB4 Ab-1510` 或 `Integrin beta4 antibody [应用技术](如IHC/WB)`。
- 部分文献可能来自抗体供应商(如CST)官网的“参考文献”栏目，可查询产品编号Ab-1510的引用列表。
The ITGB4 (Integrin Beta-4) antibody, specifically clone Ab-1510. targets the β4 subunit of integrins, a family of transmembrane adhesion receptors critical for cell-matrix interactions. Integrin β4 pairs exclusively with the α6 subunit to form α6β4. a receptor for laminins in the extracellular matrix. Unlike other integrin β subunits, β4 contains a uniquely long cytoplasmic domain that interacts with intermediate filaments via plectin, facilitating stable adhesion complexes called hemidesmosomes. These structures are vital for epithelial tissue integrity, particularly in skin, mucous membranes, and certain glands.
ITGB4 plays dual roles in cellular behavior. In normal physiology, it supports epithelial anchorage and survival. However, dysregulated ITGB4 expression or signaling is implicated in cancer progression, including enhanced tumor invasion, metastasis, and resistance to apoptosis. Overexpression of α6β4 is observed in aggressive carcinomas, such as breast, lung, and pancreatic cancers, where it promotes pro-migratory signaling through crosstalk with growth factor receptors.
The Ab-1510 antibody is commonly used in research to detect ITGB4 in applications like Western blotting, immunohistochemistry, and immunofluorescence. It helps elucidate ITGB4's role in cancer biology, epithelial homeostasis, and diseases like epidermolysis bullosa, where hemidesmosome defects cause skin fragility. By studying ITGB4 dynamics, researchers aim to uncover therapeutic targets for metastasis inhibition or tissue regeneration strategies.
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