Rabbit Polyclonal Smad1(Ab-187) Antibody

The Smad1 (Ab-187) antibody is a widely used research tool designed to detect Smad1. a key intracellular signaling protein in the bone morphogenetic protein (BMP) pathway. Smad1 belongs to the receptor-regulated Smad (R-Smad) family, which mediates BMP signal transduction from cell surface receptors to the nucleus. Upon BMP ligand binding, BMP receptors phosphorylate Smad1 at specific C-terminal serine residues (e.g., Ser463/465 in humans, corresponding to Ser187 in certain isoforms or species), enabling its association with Smad4 and subsequent nuclear translocation to regulate target gene expression. The Ab-187 clone specifically recognizes the phosphorylated form of Smad1 at Ser187 (p-Smad1), a critical post-translational modification indicative of pathway activation. This antibody is typically produced in rabbits using synthetic phosphopeptide antigens corresponding to the conserved phosphorylation site. It has been validated for applications such as Western blotting, immunoprecipitation, and immunohistochemistry in human, mouse, and rat samples. Researchers employ this antibody to study BMP signaling dynamics in developmental processes, osteogenesis, and diseases like cancer or fibrosis. Proper controls, including ligand-stimulated samples and phosphorylation site mutants, are recommended to confirm specificity due to potential cross-reactivity with other phosphorylated Smad proteins. Storage typically involves aliquoting at -20°C to avoid repeated freeze-thaw cycles.