WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 1/100-1/500 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase |
Entrez GeneID | 7083; |
WB Predicted band size | 25kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Synthesized non-phosphopeptide derived from human TK around the phosphorylation site of serine 13 (P-G-S(p)-P-S). |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于TK (Ab-13)抗体的3篇虚构参考文献示例(仅供格式参考):
---
1. **文献名称**:*Thymidine Kinase 1 as a Biomarker in Breast Cancer: Validation of Anti-TK (Ab-13) Antibody for Immunohistochemical Analysis*
**作者**:Smith, J. et al.
**摘要**:本研究验证了TK (Ab-13)抗体在乳腺癌组织中的特异性,证实其通过免疫组化检测TK1表达的可靠性,表明TK1水平与肿瘤增殖活性正相关。
2. **文献名称**:*Development of a Novel ELISA Using TK (Ab-13) for Serum TK1 Detection in Lymphoma Patients*
**作者**:Lee, H. & Garcia, R.
**摘要**:作者利用TK (Ab-13)抗体开发了一种高灵敏度的ELISA方法,成功检测淋巴瘤患者血清中的TK1水平,为疾病预后提供了新工具。
3. **文献名称**:*Comparative Study of TK Antibody Clones in Cell Cycle Research: Focus on Ab-13 Specificity*
**作者**:Chen, L. et al.
**摘要**:该研究对比了多种TK抗体克隆(包括Ab-13)在流式细胞术中的表现,发现Ab-13在识别S期细胞中TK1蛋白的稳定性和特异性最佳。
---
**注**:以上文献为示例性质,实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar搜索关键词“Thymidine Kinase Ab-13 antibody”获取准确文献。
The TK (Ab-13) antibody is a monoclonal antibody primarily used to detect thymidine kinase (TK), an enzyme critical in nucleotide metabolism and DNA synthesis. Thymidine kinase exists in two isoforms: TK1. a cytosolic enzyme expressed during the cell cycle's S-phase, and TK2. a mitochondrial isoform involved in mitochondrial DNA maintenance. Ab-13 specifically targets TK1. which is tightly linked to cellular proliferation. Elevated TK1 levels are observed in rapidly dividing cells, including cancer cells, making it a biomarker for malignancies and a potential therapeutic target.
Originally developed for research applications, the Ab-13 antibody has been utilized in techniques like ELISA, Western blotting, and immunohistochemistry to study cell cycle dynamics, tumor progression, and response to chemotherapy. Its specificity for TK1 allows researchers to distinguish proliferating cells from quiescent ones, aiding in cancer diagnostics and prognosis.
Interest in TK1 has also extended to virology, as some herpesviruses encode viral TK enzymes for nucleotide salvage, though Ab-13 does not cross-react with viral TK. Recent studies explore its role in liquid biopsies, where TK1 levels in serum correlate with tumor burden. While primarily a research tool, Ab-13's diagnostic and therapeutic potential continues to evolve, reflecting broader efforts to harness cell-cycle biomarkers in precision oncology.
×