| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CD31; CD31 antigen; EndoCAM; PEC1; PECA1 |
| Entrez GeneID | 5175; |
| WB Predicted band size | 82kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized non-phosphopeptide derived from human PECAM-1 around the phosphorylation site of tyrosine 713 (T-V-Y(p)-S-E). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PECAM-1 (Ab-713)抗体的参考文献摘要(注：Ab-713为特定克隆号，以下为模拟文献示例，实际文献需根据具体数据库检索验证)：
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1. **标题**: *"PECAM-1 regulates leukocyte transmigration by modulating endothelial cell adhesion"*
**作者**: Muller WA, et al.
**摘要**: 研究利用PECAM-1 (Ab-713)抗体阻断实验，证明PECAM-1在白细胞跨内皮迁移中的关键作用，通过调控内皮细胞间粘附连接影响炎症反应。
2. **标题**: *"Role of PECAM-1 in angiogenesis: Insights from antibody-mediated inhibition"*
**作者**: DeLisser HM, et al.
**摘要**: 使用Ab-713抗体抑制PECAM-1功能，发现其通过干扰内皮细胞间信号传导抑制血管生成，提示其在肿瘤微血管形成中的潜在治疗价值。
3. **标题**: *"PECAM-1 phosphorylation and SHP-2 activation in platelet aggregation"*
**作者**: Jackson DE, et al.
**摘要**: 通过Ab-713抗体免疫沉淀技术，揭示PECAM-1酪氨酸磷酸化招募SHP-2磷酸酶，负向调控血小板活化通路，防止过度血栓形成。
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如需具体文献，建议通过PubMed或Web of Science以“PECAM-1 CD31 Ab-713”为关键词检索，并筛选目标克隆号的应用研究。
PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1), also known as CD31. is a 130-kDa transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. It is predominantly expressed on endothelial cells, platelets, and certain immune cells, playing critical roles in vascular homeostasis, leukocyte transmigration, and angiogenesis. PECAM-1 contains six extracellular Ig-like domains, a transmembrane region, and a cytoplasmic tail with signaling motifs. It mediates cell-cell adhesion via homophilic interactions and participates in intracellular signaling pathways regulating inflammation and vascular permeability.
The PECAM-1 (Ab-713) antibody is a specific reagent targeting a phosphorylated tyrosine residue (Tyr713) within the cytoplasmic domain of human PECAM-1. This phosphorylation site is associated with downstream signaling events, including the recruitment of SHP-2 phosphatase, which modulates cellular responses to shear stress and inflammatory stimuli. The antibody is widely used in immunoblotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) to study PECAM-1 activation, vascular biology, and pathological processes like atherosclerosis or tumor angiogenesis. Its specificity for the phosphorylated form makes it valuable for investigating post-translational modifications of PECAM-1 in endothelial dysfunction or leukocyte-endothelial interactions.
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