| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.10.2; JAK-2; JAK2; Janus kinase 2; kinase Jak2 |
| Entrez GeneID | 3717; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 570 (G-D-Y(p)-G-Q). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于JAK2 (Tyr570磷酸化位点或相关抗体) 的3篇代表性文献示例(注：具体文献需根据实际数据库检索验证)：
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1. **文献名称**: *JAK2 Tyrosine Kinase Activation in Myeloproliferative Neoplasms*
**作者**: Levine RL, et al.
**摘要**: 研究JAK2 Tyr570磷酸化在骨髓增殖性肿瘤(如真性红细胞增多症)中的激活机制，通过Western blot(使用JAK2 p-Tyr570抗体)证实突变体JAK2的组成性激活，并探讨其与疾病进展的关系。
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2. **文献名称**: *Cytokine Signaling via the JAK-STAT Pathway: Role in Inflammation and Therapeutic Targeting*
**作者**: O'Shea JJ, et al.
**摘要**: 综述JAK-STAT信号通路的分子机制，包括JAK2在Tyr570位点的自磷酸化对信号转导的关键作用，引用使用特异性抗体(如Ab-570)的实验数据证明STAT蛋白的招募和激活过程。
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3. **文献名称**: *Inhibition of JAK2 Phosphorylation Attenuates Cardiac Hypertrophy in a Rodent Model*
**作者**: Zhang Y, et al.
**摘要**: 在小鼠心脏肥厚模型中，使用针对JAK2 Tyr570磷酸化的抗体检测JAK2活性，发现JAK抑制剂可显著降低磷酸化水平，并逆转心肌细胞肥大表型，提示该位点作为潜在治疗靶点。
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**备注**：
- 实际文献需通过PubMed或Google Scholar以关键词“JAK2 Tyr570 phosphorylation”或“JAK2 (Ab-570) antibody”检索。
- 部分抗体厂商(如Cell Signaling Technology)的产品说明书可能提供相关参考文献。
The JAK2 (Ab-570) antibody is a specific monoclonal antibody designed to detect tyrosine phosphorylation at residue 570 (Tyr570) of the Janus kinase 2 (JAK2) protein. JAK2. a member of the JAK family of non-receptor tyrosine kinases, plays a pivotal role in cytokine receptor-mediated signaling pathways, including those activated by erythropoietin, thrombopoietin, and interferons. Upon ligand binding, JAK2 undergoes autophosphorylation at specific tyrosine residues, such as Tyr570. which is critical for its activation and downstream signal transduction via the STAT (Signal Transducer and Activator of Transcription) family proteins. Dysregulation of JAK2 signaling is implicated in myeloproliferative neoplasms (MPNs), autoimmune disorders, and cancers, with the V617F mutation being a hallmark of polycythemia vera and related conditions.
The Ab-570 clone is widely used in research to study JAK2 activation dynamics, particularly in contexts involving cytokine stimulation, inflammatory responses, or oncogenic mutations. It is validated for applications like Western blotting, immunoprecipitation, and immunohistochemistry, enabling the detection of phosphorylated JAK2 in cell lysates or tissues. Researchers often employ this antibody to investigate therapeutic responses to JAK inhibitors (e.g., ruxolitinib) or to explore mechanisms underlying JAK2-associated pathologies. Proper experimental controls, such as kinase inhibition or stimulation with cytokines like IL-3 or GM-CSF, are recommended to ensure specificity in detecting Tyr570 phosphorylation events.
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