| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Signal transducer and activator of transcription 5B; |
| Entrez GeneID | 6777; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human STAT5B around the phosphorylation site of serine 731(A-P-S-P-A). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于STAT5B (Ab-731)抗体的3篇示例参考文献(内容为虚构，仅供参考，需结合实际文献验证)：
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1. **文献名称**：*STAT5B Activation in T-cell Leukemia Mediates Oncogenic Signaling*
**作者**：Smith J, et al. (2018)
**摘要**：本研究利用STAT5B (Ab-731)抗体进行Western blot和免疫共沉淀实验，揭示了STAT5B的持续磷酸化在T细胞白血病中的关键作用，并发现其与JAK2激酶的异常激活相关。
2. **文献名称**：*Growth Hormone-Induced STAT5B Nuclear Translocation in Liver Regeneration*
**作者**：Zhang Y, et al. (2020)
**摘要**：通过免疫组化(使用STAT5B Ab-731抗体)和ChIP-seq技术，作者证明生长激素通过STAT5B调控肝脏再生相关基因的表达，并揭示了其与代谢稳态的关联。
3. **文献名称**：*STAT5B Dysregulation in Autoimmune Disorders: Evidence from Patient-Derived Samples*
**作者**：Brown K, et al. (2019)
**摘要**：该研究采用STAT5B (Ab-731)抗体分析类风湿性关节炎患者的组织样本，发现STAT5B的异常表达与自身免疫反应的过度激活显著相关，提示其作为潜在治疗靶点。
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**注意**：以上文献为示例，实际引用时需通过PubMed、Google Scholar等平台检索关键词“STAT5B antibody Ab-731”或联系抗体供应商(如CST、Abcam)获取引用文献列表。部分厂商官网会提供该抗体在已发表研究中的具体应用案例。
The STAT5B (Ab-731) antibody is a widely used tool in molecular biology research to study the Signal Transducer and Activator of Transcription 5B (STAT5B) protein, a key mediator of cytokine and growth factor signaling. STAT5B, along with its homolog STAT5A, belongs to the STAT protein family and is activated through phosphorylation in response to extracellular signals, such as interleukins, prolactin, and growth hormones. Once phosphorylated (typically at Tyr694/699), STAT5B dimerizes, translocates to the nucleus, and regulates the transcription of target genes involved in cell proliferation, differentiation, and immune responses. Dysregulation of STAT5B is linked to cancers, autoimmune disorders, and metabolic diseases.
The STAT5B (Ab-731) antibody specifically recognizes endogenous STAT5B protein, often differentiating it from STAT5A due to subtle sequence differences. It is commonly employed in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to assess STAT5B expression, activation status, and subcellular localization. Researchers use this antibody to investigate STAT5B's role in signaling pathways, particularly in immune cells, hematopoietic systems, and cancer models. Its reliability in detecting both phosphorylated and non-phosphorylated forms (depending on the specific product variant) makes it valuable for studying pathway dynamics. Validation typically includes testing in knockout models or siRNA-treated cells to confirm specificity. This antibody has contributed to studies exploring STAT5B's involvement in diseases like leukemia, lymphoma, and immune dysregulation syndromes.
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