| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AGMX1; ATK; Agammaglobulinaemia tyrosine kinase; B cell progenitor kinase; BPK |
| Entrez GeneID | 695; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human BTK around the phosphorylation site of tyrosine 222 (A-L-Y(p)-D-Y). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于BTK (Ab-222)抗体的示例参考文献(注：以下内容为模拟示例，具体文献需根据实际研究核实)：
---
1. **文献名称**：*BTK Signaling in B Cell Development and Autoimmunity*
**作者**：Smith A, et al.
**摘要**：该研究使用BTK (Ab-222)抗体通过Western blot和免疫沉淀技术，分析BTK在B细胞受体信号通路中的活化机制，证明其在自身免疫性疾病模型中异常激活。
2. **文献名称**：*Targeting BTK in Chronic Lymphocytic Leukemia*
**作者**：Lee C, et al.
**摘要**：作者利用BTK (Ab-222)抗体进行免疫组化实验，检测白血病患者组织中BTK蛋白的表达水平，揭示其与药物耐药性的相关性。
3. **文献名称**：*Phosphorylation-Dependent Regulation of BTK Activity*
**作者**：Wang Y, et al.
**摘要**：研究通过BTK (Ab-222)抗体结合流式细胞术，验证BTK酪氨酸磷酸化在B细胞淋巴瘤中的功能，为靶向治疗提供依据。
4. **文献名称**：*BTK Inhibitors and Immune Modulation*
**作者**：Zhang R, et al.
**摘要**：该文献使用BTK (Ab-222)抗体进行小鼠模型组织染色，证明抑制BTK可调节巨噬细胞炎症反应，拓展了其在免疫疾病中的应用场景。
---
**注意**：以上文献信息为示例，实际研究中需根据具体抗体来源(如CST、Abcam等厂商的克隆号)和实验背景检索真实文献。建议通过PubMed或Web of Science以关键词“BTK antibody Ab-222”或“anti-BTK clone 222”进一步筛选。
The Bruton's tyrosine kinase (BTK) antibody, specifically the clone Ab-222. is a monoclonal antibody designed to detect and study BTK, a non-receptor tyrosine kinase critical in B-cell development and signaling. BTK plays a pivotal role in the B-cell receptor (BCR) signaling pathway, regulating proliferation, differentiation, and survival. Mutations in the *BTK* gene are linked to X-linked agammaglobulinemia (XLA), a primary immunodeficiency disorder characterized by impaired B-cell maturation.
The Ab-222 antibody is commonly used in research to investigate BTK expression and function in normal and pathological contexts, including B-cell malignancies (e.g., chronic lymphocytic leukemia, mantle cell lymphoma) and autoimmune diseases. It binds to a specific epitope of human BTK, enabling applications such as Western blotting, immunohistochemistry, and flow cytometry.
Developed as a tool for both basic and translational research, Ab-222 aids in studying BTK inhibitors (e.g., ibrutinib) and their therapeutic mechanisms. Its specificity and reliability make it valuable for monitoring BTK expression levels in clinical samples, drug development, and understanding resistance mechanisms in targeted therapies. The antibody’s utility extends to exploring BTK’s role in innate immune signaling and its cross-talk with other pathways, underscoring its importance in immunology and oncology research.
×
