| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2 |
| Entrez GeneID | 3326; |
| WB Predicted band size | 92kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-S(p)-D-E). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HSP90B (Ab-254)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**: *GRP94 in Cancer: Molecular Mechanisms and Therapeutic Potential*
**作者**: Lee AS, et al.
**摘要**: 研究揭示了内质网伴侣蛋白GRP94(HSP90B1)在肿瘤生长和转移中的作用，利用Ab-254抗体通过免疫沉淀和免疫组化验证其在癌细胞中的高表达，并表明靶向GRP94可抑制肿瘤微环境中的促癌信号通路。
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2. **文献名称**: *Endoplasmic Reticulum Stress and GRP94 in Autoimmune Diseases*
**作者**: Zhang K, et al.
**摘要**: 探讨GRP94在自身免疫性疾病中的调控机制，通过Ab-254抗体进行Western blot和流式细胞术分析，发现其通过激活树突状细胞中的TLR信号通路促进炎症反应，为治疗提供新靶点。
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3. **文献名称**: *Role of GRP94 in Protein Quality Control and Neurodegeneration*
**作者**: Wang M, et al.
**摘要**: 研究GRP94在神经退行性疾病(如阿尔茨海默病)中的功能，使用Ab-254抗体检测其与错误折叠蛋白的相互作用，证实其通过内质网相关降解(ERAD)途径参与清除致病性蛋白聚集体。
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**备注**：上述文献中使用的Ab-254抗体通常指向针对HSP90B1/GRP94的特定克隆抗体(如Cell Signaling Technology的#2543)，需结合具体实验背景确认。若需全文或更多文献，建议通过PubMed或Sci-Hub等平台检索对应标题。
The HSP90B (Ab-254) antibody targets a specific epitope within the HSP90B protein, also known as glucose-regulated protein 94 (GRP94), a member of the heat shock protein 90 (HSP90) family. HSP90B1 is an endoplasmic reticulum (ER)-resident molecular chaperone critical for protein folding, quality control, and stress response. Unlike its cytosolic paralogs (HSP90α/β), HSP90B1 operates exclusively in the ER, assisting in the maturation of secretory and membrane proteins, including immunoglobulins, Toll-like receptors (TLRs), and integrins. It plays a role in ER stress pathways, interacting with key sensors like PERK and IRE1α during the unfolded protein response (UPR).
The Ab-254 antibody is commonly used in Western blotting, immunoprecipitation, and immunofluorescence to study HSP90B1 expression, localization, and function in human, mouse, and rat samples. Its specificity for the 254th amino acid region ensures reliable detection of the full-length protein (~94 kDa). Research highlights HSP90B1's involvement in cancer progression, autoimmune disorders, and neurodegenerative diseases, where its overexpression correlates with poor prognosis or pathological protein aggregation. The antibody serves as a tool to explore HSP90B1's role as a therapeutic target or biomarker in ER stress-related conditions.
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