| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | histone deacetylase 7, HD7A, HDAC7A |
| Entrez GeneID | 51564; |
| WB Predicted band size | 66kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to a region derived from 938-951 amino acids of Human histone deacetylase 7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC7抗体的3篇代表性文献的简要信息(注：文献标题和作者为模拟示例，实际需根据真实文献调整)：
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1. **标题**: "HDAC7 Regulates Vascular Endothelial Cell Function via Epigenetic Modulation"
**作者**: Smith A, et al.
**摘要**: 研究通过Western blot和免疫荧光技术，利用HDAC7特异性抗体，发现HDAC7通过去乙酰化作用调控血管内皮细胞的迁移和增殖，影响血管生成过程。
2. **标题**: "Role of HDAC7 in T-cell Apoptosis and Autoimmunity"
**作者**: Lee B, et al.
**摘要**: 通过流式细胞术和ChIP实验(使用HDAC7抗体)，揭示HDAC7在T细胞凋亡中的关键作用，其缺失导致自身免疫反应增强，提示其作为治疗靶点的潜力。
3. **标题**: "HDAC7 Antibody-Based Detection in Cancer Cell Lines Reveals Expression Heterogeneity"
**作者**: Chen L, et al.
**摘要**: 利用商业化HDAC7抗体对多种癌细胞系进行免疫组化分析，发现HDAC7表达水平与肿瘤侵袭性相关，为癌症分型提供潜在生物标志物。
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**注意**：以上文献为示例，实际引用需通过PubMed、Google Scholar等平台检索真实文献(关键词：HDAC7 antibody, Western blot, ChIP等)。如需具体文献，建议提供更详细的研究背景或应用场景。
**Background of HDAC7 Antibodies**
Histone deacetylase 7 (HDAC7) is a class IIa HDAC enzyme involved in epigenetic regulation by removing acetyl groups from histone proteins, leading to chromatin condensation and transcriptional repression. It plays critical roles in cellular processes such as differentiation, proliferation, apoptosis, and immune regulation, particularly in T-cell development and vascular endothelial function. HDAC7 is also implicated in diseases, including cancer, cardiovascular disorders, and metabolic syndromes.
HDAC7 antibodies are essential tools for detecting and studying this protein in research. They enable the identification of HDAC7 expression levels, subcellular localization (shuttling between nucleus and cytoplasm), and interactions with binding partners (e.g., transcription factors or other HDACs). These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP).
Due to structural similarities among HDAC isoforms, HDAC7 antibodies must exhibit high specificity to avoid cross-reactivity. Validation using HDAC7 knockout controls or siRNA-mediated knockdown is critical. Commercial HDAC7 antibodies are often raised against unique epitopes within its N-terminal regulatory domain or C-terminal catalytic region. Their applications extend to studying HDAC7’s role in signaling pathways (e.g., TGF-β, MAPK) and evaluating therapeutic HDAC inhibitors in disease models.
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