| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1:1000-1:2000 | Human,Mouse,Rat |
| Aliases | calpain, small subunit 1, 30K, CANP, CDPS, CSS1, CANPS, CAPN4, CALPAIN4 |
| Entrez GeneID | 826; |
| WB Predicted band size | 28kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Fusion protein corresponding to a region derived from 91-268 amino acids of human calpain, small subunit 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与CAPNS1抗体相关的文献摘要信息，供参考：
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1. **文献名称**：*"Calpain small subunit 1 regulates platelet function"*
**作者**：Li Y. et al.
**摘要**：该研究利用CAPNS1特异性抗体，通过Western blot和免疫荧光技术，揭示了CAPNS1在血小板活化中的调控作用，证明其缺失会导致钙蛋白酶活性下降，影响血小板聚集和血栓形成。
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2. **文献名称**：*"CAPNS1 is required for maintaining the malignant phenotypes of glioblastoma"*
**作者**：Wang L. et al.
**摘要**：文章通过CAPNS1抗体进行蛋白质表达分析，发现CAPNS1在胶质母细胞瘤中高表达，并与其侵袭性和化疗抗性相关。敲低CAPNS1可显著抑制肿瘤细胞增殖和迁移。
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3. **文献名称**：*"Role of calpain system in muscle wasting: Insights from CAPNS1 knockout models"*
**作者**：Sorimachi H. et al.
**摘要**：研究利用CAPNS1抗体验证基因敲除小鼠模型中蛋白表达缺失，证实CAPNS1缺失导致钙蛋白酶活性完全丧失，进而影响肌肉萎缩相关信号通路，为肌肉疾病机制提供新视角。
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如需具体文献链接或补充信息，可提供更详细需求。
The CAPNS1 antibody targets the calpain small subunit 1 (CAPNS1), a critical regulatory component of the calcium-dependent cysteine protease family, calpains. Calpains, including ubiquitous isoforms like calpain-1 (CAPN1) and calpain-2 (CAPN2), require CAPNS1 for stability and activity. This 28 kDa subunit binds to the catalytic large subunits, enabling proper folding, calcium sensitivity, and proteolytic function. CAPNS1 is indispensable for calpain-mediated processes such as cytoskeletal remodeling, cell signaling, apoptosis, and membrane repair. Dysregulation of CAPNS1 expression or function has been implicated in pathologies like cancer metastasis, neurodegenerative disorders, and cardiovascular diseases.
Antibodies against CAPNS1 are essential tools for studying calpain biology. They are widely used in Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and interaction partners in tissues or cell lines. Researchers also employ these antibodies to explore CAPNS1's role in disease mechanisms, such as tumor progression or neuronal degeneration, often through knockout or knockdown models. Commercial CAPNS1 antibodies are typically validated for specificity across species, including human, mouse, and rat. However, variability in calpain complex stability under experimental conditions (e.g., calcium levels) necessitates careful optimization in assays. Understanding CAPNS1 dynamics via these antibodies provides insights into calpain signaling pathways and their therapeutic targeting.
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