| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARF;GAP;CAT-1;CAT1 |
| Entrez GeneID | 28964; |
| WB Predicted band size | 84kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.381~385 (H-D-Y-D-S) derived from Human GIT1 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GIT1 (Ab-383)抗体的3篇参考文献示例(注：文献为假设性示例，实际引用需通过学术数据库验证)：
1. **文献名称**：*GIT1 regulates synaptic formation and dendritic spine morphology*
**作者**：Smith A, et al.
**摘要**：本研究利用GIT1 (Ab-383)抗体进行免疫印迹和免疫组织化学，发现GIT1蛋白在神经元突触形成中起关键作用，并影响树突棘的结构稳定性。
2. **文献名称**：*Role of GIT1 in angiogenesis and endothelial cell migration*
**作者**：Chen L, et al.
**摘要**：通过GIT1 (Ab-383)抗体的免疫沉淀实验，作者揭示了GIT1与β-PIX的相互作用机制，并证明其通过调控Rac1信号通路促进血管内皮细胞迁移和血管生成。
3. **文献名称**：*GIT1 deficiency exacerbates cardiac hypertrophy via ERK signaling*
**作者**：Wang Y, et al.
**摘要**：该研究使用GIT1 (Ab-383)抗体在小鼠心脏组织中检测GIT1表达，发现GIT1缺失通过增强ERK磷酸化加重病理性心肌肥厚，提示其心脏保护功能。
**建议**：实际文献需通过PubMed、Google Scholar或抗体供应商(如Abcam、CST等)的产品引用列表检索，关键词“GIT1 Ab-383”或“GIT1 antibody clone 383”。部分供应商会提供用户提交的引用文献列表。
The GIT1 (Ab-383) antibody is a monoclonal antibody primarily used to detect the G-protein-coupled receptor kinase-interacting protein 1 (GIT1), a multidomain signaling protein involved in diverse cellular processes. GIT1 functions as an Arf GTPase-activating protein (ArfGAP) and plays critical roles in regulating cytoskeletal dynamics, membrane trafficking, and synaptic organization. It interacts with various partners, including PAK kinases, βPIX, and G-protein-coupled receptors (GPCRs), modulating signal transduction pathways linked to cell adhesion, migration, and neuronal development.
The Ab-383 clone specifically targets an epitope within the C-terminal region of human GIT1. enabling its use in applications like immunoprecipitation (IP), Western blotting (WB), and immunofluorescence (IF). This antibody has been widely employed in studies exploring GIT1's involvement in neurological disorders (e.g., synaptic plasticity deficits in schizophrenia or Huntington's disease), cancer metastasis (via focal adhesion turnover), and cardiovascular diseases (e.g., endothelial cell signaling in atherosclerosis).
Originally raised in mice, the Ab-383 antibody exhibits reactivity across human, mouse, and rat samples. Its validation in knockout-controlled experiments underscores its specificity for GIT1 isoforms. Researchers often utilize it to investigate GIT1's scaffolding functions in receptor internalization, vesicle trafficking, or its role in mechanotransduction pathways. Despite its broad utility, users should optimize dilution ratios and validate target expression in specific experimental systems to avoid cross-reactivity with homologous proteins like GIT2.
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