| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cofilin; non-muscle isoform 18 kDa phosphoprotein; |
| Entrez GeneID | 1072; |
| WB Predicted band size | 19kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 86~90 (A-T-Y-E-T) derived from Human cofilin1/cofilin2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与cofilin1/cofilin2(Ab-88)抗体相关的参考文献示例(内容基于假设性研究)：
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1. **文献名称**: *"Phosphorylation of cofilin1 at Ser88 regulates actin dynamics in neuronal growth cones"*
**作者**: Smith A, et al.
**摘要**: 研究利用cofilin1(Ab-88)特异性抗体，证实Ser88磷酸化通过抑制cofilin1的肌动蛋白解聚活性，影响神经元轴突导向和突触可塑性。
2. **文献名称**: *"Cofilin2 phosphorylation at Ser88 modulates cardiac muscle contraction"*
**作者**: Chen L, et al.
**摘要**: 通过Ab-88抗体检测发现，cofilin2的Ser88磷酸化水平在心肌缺血时显著升高，导致肌动蛋白稳定性改变，进而影响心脏收缩功能。
3. **文献名称**: *"Role of cofilin1 Ser88 phosphorylation in cancer cell migration and invasion"*
**作者**: Tanaka K, et al.
**摘要**: 使用Ab-88抗体分析发现，EGFR信号通路通过激活LIM激酶(LIMK)诱导cofilin1 Ser88磷酸化，促进肿瘤细胞侵袭转移。
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**注**：以上文献为假设示例，实际研究中需通过数据库(如PubMed)检索具体抗体使用文献。若需真实文献，建议以“cofilin1 phosphorylation Ser88 antibody”或抗体货号(如CST #3312)为关键词进一步查询。
Cofilin1 and cofilin2 are actin-binding proteins critical for regulating cytoskeletal dynamics by severing and depolymerizing filamentous actin (F-actin). While cofilin1 (non-muscle cofilin) is ubiquitously expressed in non-muscle cells, cofilin2 is predominantly found in skeletal and cardiac muscle. Both isoforms share ~70% sequence homology but exhibit distinct regulatory mechanisms and tissue-specific functions. Their activity is modulated by phosphorylation at Ser-3. which inhibits actin binding, and dephosphorylation reactivates their F-actin disassembly function. Dysregulation of cofilin signaling is linked to pathologies such as cancer metastasis, neurodegenerative disorders, and muscle diseases.
The cofilin1/cofilin2 (Ab-88) antibody is a research tool designed to recognize both isoforms, typically targeting a conserved epitope that includes phosphorylated Ser-3. This antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study cofilin expression, localization, and post-translational modifications. Its ability to detect phosphorylated forms (inactive state) allows researchers to explore dynamic cytoskeletal changes during processes like cell migration, synaptic plasticity, or muscle contraction. Validation often includes testing in isoform-specific knockout models to confirm cross-reactivity. As cofilin dysfunction is implicated in diseases like Alzheimer's and muscular dystrophy, Ab-88 serves as a key reagent for dissecting cytoskeletal mechanisms in both physiological and pathological contexts.
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